分化型甲状腺癌组织中存活素蛋白的表达与细胞增殖和凋亡的关系

背景与目的： 探讨分化型甲状腺癌(differentiated thyroid carcinoma, DTC)组织中存活素(Survivin)蛋白的表达与细胞增殖和凋亡活性的关系，以及在甲状腺癌发生、发展过程中的作用机制及可能的作用途径。 材料与方法： 采用末端脱氧核苷酸转移酶标记(TUNEL)法及免疫组化技术分别对10例结节性甲状腺肿(结甲)、10例甲状腺滤泡状腺瘤、30例甲状腺乳头状癌、12例甲状腺滤泡状癌组织中细胞凋亡指数(apoptosis index,AI)、细胞增殖核抗原增殖指数(PCNA-LI, PI)和Survivin蛋白的表达进行检测。 结果： 甲状腺癌组中Survivin蛋白的表达阳性率均显著高于对照组结甲和腺瘤中的阳性表达率；甲状腺癌组织中Survivin的表达与淋巴结转移和临床分期有关(P<0.05)；且Survivin蛋白表达阳性组和阴性组的AI和PI均呈负相关(r分别为－0.72和－0.80，P均<0.01)；分别比较Survivin蛋白表达阳性组和阴性组的AI及PI，两组间AI和PI的差异均具有统计学意义(P<0.01)。 结论： Survivin与分化型甲状腺癌细胞增殖和凋亡关系密切，在甲状腺癌发生发展过程中发挥重要的作用。

The Expression of Survivin and Its Relationship with Cellular Proliferation and Apoptosis in Differentiated Thyroid Carcinoma

BACKGROUND AND AIM: To investigate the expression of Survivin protein and its relationship with cellular proliferation and apoptosis in differentiated thyroid carcinoma(DTC),and to identify the mechanism and pathway of Survivin in thyroid tumorigenesis. MATERIALS AND METHODS: The terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick end labeling (TUNEL) technique was used to measure the apoptosis index(AI). Expression of Survivin protein and proliferating cell nuclear antigen(PCNA) in 10 cases of nodular goiters (NG),10 thyroid adenomas (TA),30 papillary thyroid carcinoma(PTC) and 12 follicular thyroid carcinoma (FTC) were determined by immunohistochemistry staining. RESULTS: The positive staining rate of Survivin in thyroid carcinoma was higher than that in control group (P<0.01). The expression of Survivin in thyroid carcinoma was related to lymph node metastasis and clinical stage(P<0.05). In DTC group AI was negatively related with the PI in patients with positive survivin expression and in patients with negative Survivin expression(r=-0.72,r=-0.80,P<0.01),respectively.In DTC,the PI in patients with positive Survivin expression was higher than that in patients with negative Survivin expression. However,the AI in patients with positive Survivin expression was lower than that in patients with negative Survivin expression(P<0.01). CONCLUSION: Survivin demonstrated a closed relationship with cellular proliferation and apoptosis in differentiated thyroid carcinoma.It may play an important role in the development of thyroid carcinoma .