人类Vimentin基因第1,2,6,7内含子序列的测定

目的]人vimentin(VIM)基因位于10号染色体短臂1区3带,属于第Ⅲ型中间丝纤维蛋白基因.目前已知全部外显子序列及部分内含子序列,本文测定了部分未知的VIM基因内含子序列.方法]通过已知VIM基因的外显子序列设计引物,用PCR扩增其未知的内含子片段,将PCR产物进行克隆测序.结果]共测得未知的4个内含子序列,内含子1长692bp,测得其全部序列;内含子2长2kb,测得其两端与外显子相接的序列,共1kb;内含子6长456bp,测得其全部序列;内含子7长404 bp,测得其全部序列.结论]本研究PCR检测多例人VIM基因的内含子7大小为404 bp,与文献报道人类vi-mentin基因的内含子7大小为800bp相差很大,但引物两端外显子序列相同.另外,文献报道的人类vimentin基因与外显子3相邻的第2内含子末端41nt的核苷酸序列与本研究所测的第2内含子末端序列完全不同.

Introns 1,2,6,7 Sequencing of the Human Vimentin Gene

Human vimentin(VIM) gene is located on the chromosome 10p13, belonging to the third type intermediate filament protein genes. Now the nucleotide sequence of all exons and part of introns of vimentin gene has already been known. Some unknown introns of vimentin gene were sequenced in this study. According to the sequence of VIM gene exon, primers were designed and the unknown introns were amplified by using PCR. Then the title segments were cloned and sequenced. Four unknown introns were sequenced: intron 1 was 692 bp, the sequence was all identified; intron 2 was 2 kb or so, the two side of the sequence was identified; intron 6 was 456 bp, the sequence was all identified; intron 7 was 404 bp, the sequence was all identified. Conclusion] The length of VIM gene intron 7 is 404 bp, different from that of 800 bp reported in literature. The end 41 nt sequence of VIM gene intron 2 near exon 3 is also different from the sequence reported in literature.