PTEN/PI3K信号转导途径与子宫内膜癌生物学行为的相关性

目的 :研究PTEN/PI3K信号转导途径与子宫内膜癌发生发展的关系。方法 :应用免疫组化和免疫印迹方法对 68例子宫内膜癌和癌前病变组织及 11例正常子宫内膜进行PTEN、p PKB及p Bad蛋白检测。结果 :1)PTEN蛋白的阳性表达率在正常增殖期子宫内膜组织中最高 ( 90 9% ) ,非典型增生组织中开始下降 ,在子宫内膜癌组织中明显降低 ( 4 9 1% ) ,三者之间比较 ,差异有统计学意义 ,P <0 0 1;2 )p PKB及p Bad与PTEN的阳性表达呈相反趋势 ,在非典型增生组织及内膜癌组织中p PBK及p Bad表达阳性率明显增加 ,差异均有统计学意义 ,P <0 0 1,P <0 0 5。在子宫内膜癌PTEN表达阴性组织中p PKB、p Bad蛋白染色积分均明显高于PTEN阳性组 ,P <0 0 5 ,P <0 0 1;3 )相关分析显示PTEN与p PKB及p Bad表达均呈负相关 ,r =-0 67,P <0 0 5 ;r =-0 65 ,P <0 0 5 ;4)PTEN、p Bad蛋白的阳性表达率在不同的临床分期、组织学分级及不同肌层浸润程度之间比较 ,差异均无统计学意义 ,P >0 0 5 ,而PTEN、p PKB只在临床分期间差异有统计学意义 ,P <0 0 5。结论 :PTEN/PI3K信号转导途径与子宫内膜癌的发生密切相关。伴随PTEN表达缺失 ,p PKB、p Bad阳性表达更加明显 ,提示PTEN蛋白表达可作为子宫内膜癌早期诊断指标和基因治疗靶点。

Relationship between PTEN/PI3K pathway and biological behavior of endometrial carcinoma

OBJECTIVE:To investigate the relationship between PTEN/PI3K pathway and the occurrence, development of endometrial carcinoma. METHODS: The expression of PTEN, p-PKB and p-Bad in 68 endometrial carcinoma or precancerous tissues and 11 normal endometrium were detected by the immunohistochemistry and Western blot tests. RESULTS: 1)The highest positive rate of PTEN expression was found in normal proliferative phase endometrium.The positive rate decreased slightly in atypical hyperplasia tissues, and being obviously in endometrial carcinoma(49.1%) the difference was high significant,P<0.01. 2)The expressions of p-PKB and p-Bad were opposite to that of PTEN, their positive rates obviously increasd in atypical hyperplasia and endometrial carcinoma tissues.The differences were also significant,P<0.01,P<0.05 respectively. In endometrial carcinoma, the positive scores of p-PKB and p-Bad in PTEN negative group were higher than those of PTEN positive one significantly,P<0.05,P<0.01 respectively. 3)Correlation analysis revealed that expression of PTEN was negatively related to that of both p-PKB,r=-0.67,P<0.05 and p-Bad,r=-0.65,P<0.05. 4)There were no differences between the positive expressions of PTEN and that of p-Pad in different clinical phase,histological grade and muscle invasion.The difference of p-PKB expression was sigtnificant in different clinical phases of endometrial carcinoma,P<0.05.CONCLUSIONS:PTEN/PI3K pathway is closely related with the occurrence of endometrial carcinoma. With the failure of PTEN expression, the expressions of p-PKB and p-Bad are more intensive, so PTEN may be the marker for early diagnosis and the target for gene therapy in endometrial carcinoma.