| 微RNAlet-7d抑制胶质瘤生长和侵袭 |
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| 引用本文： | 程子昊,尤永平,石磊,刘宁. 微RNAlet-7d抑制胶质瘤生长和侵袭[J]. 中国神经肿瘤杂志, 2008, 6(3): 174-178 |
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| 作者姓名： | 程子昊 尤永平 石磊 刘宁 |
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| 作者单位： | 南京医科大学第一附属医院神经外科,江苏南京210029 |
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| 基金项目： | 国家自然科学基金项目资助（30672165）江苏省重点人才项目（RC2007061） |
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| 摘 要： | 背景与目的：let-7d与胶质瘤的关系尚不清楚,本研究探讨let-7d在人脑胶质瘤组织中表达及其对人脑胶质瘤U87细胞生物学特征的影响。方法：原位杂交法检测let-7d在人脑胶质瘤组织和正常脑组织中的表达,利用脂质体将let-7d寡核苷酸转入U87细胞,观察各组细胞生长、凋亡、侵袭情况。结果：人脑胶质瘤组织中let-7d较正常人脑组织低表达,转入let-7d寡核苷酸的U87细胞,生长减缓,凋亡增加,侵袭力减低。结论：微RNAlet-7d在胶质瘤的发生发展中可能起抑制作用。
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| 关 键 词： | 胶质瘤 微RNA let-7d 侵袭 |
MiRNA let-7d Functions as a Potential Growth Suppressor in Development and Progression of Gliomas |
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| Zi-hao Cheng,Yong-ping You,Lei Shi,Ning Liu. MiRNA let-7d Functions as a Potential Growth Suppressor in Development and Progression of Gliomas[J]. Chinese Journal of Neuro-Oncology, 2008, 6(3): 174-178 |
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| Authors: | Zi-hao Cheng Yong-ping You Lei Shi Ning Liu |
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| Affiliation: | (Department of Neurosurgery,the First Affiliated Hospital,Nanjing Medical University,Nanjing 210029,P.R.China ) |
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| Abstract: | BACKGROUND ＆ OBJECTIVE： The relationship between let-7d and gliomas is still not clear.To investigate the expression of let-7d in human glioma specimens and the influence on biological characteristics of human brain glioblastoma U87 cells. METHODS： In situ hybridization （ISH） technique was applied to detect the expression of let-7d in human glioma specimens and normal brain tissues. Let-7d oliogonucleotide was transfected to U87 cell with liposome. The growth, apoptosis and invasion of U87 cells were monitored. RESULTS： The expression level of let-7d miRNA in human brain glioma tissues was lower compared to that in normal brain tissues.The growth and invasion of U87 cells transfected with let-7d oligonucletide retarded while the amount of apoptotic cell increased. CONCLUSIONS： MiRNA let-7d may function as a potential growth suppressor in development and progression of human brain gliomas. |
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| Keywords: | Glioma MiRNA Let-7d Invasion |
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