哇巴因诱导白血病细胞凋亡的作用机制

目的：研究哇巴因对白血病细胞株Jurkat是否具有凋亡诱导作用，探索其可能的信号传导通路，阐明哇巴因诱导细胞凋亡的作用机制。方法：采用四甲基偶氮唑盐(MTT)法观察哇巴因对白血病细胞生长的抑制程度；应用电镜及流式细胞术观测细胞凋亡；应用Western-blot检测细胞外信号调节蛋白激酶(extracelluar sig-nal-regulated kinase1/2简称 ERK1/2)的表达。结果：哇巴因能诱导白血病细胞凋亡，细胞凋亡的数量与哇巴因的浓度呈正相关，Western-blot检测结果显示哇巴因作用后，磷酸化：ERK1/2的表达减低。结论：细胞外信号调节蛋白激酶ERK通路可能参与了哇巴因引起的白血病细胞的凋亡过程。

The study of molecular mechanism of ouabain-induced apoptosis in Jurkat cells

Objective:To investigate whether ouabain can induce the apoptosis of Jurkat cells and to explore the signal transduction pathway of apoptosis. Method:Using the MTT method for assaying the inhibition degree of cell proliferation; Electron microscope and flow cytometry were used to analyze apoptosis; The expression of extracelluar signal-regulated kinase 1/2(ERK 1/2) was determined by Western blot. Result:Ouabain could induce Jurkat cells towards apoptosis. The number of apoptotic cells was correlated with the concentration of ouabain. The result of Western-blot indicated that the expression of phosphorylated ERK 1/2 protein was down-regulated after treated with ouabain. Conclusion:ERK pathway may be involved in the ouabain-induced apoptosis of Jurkat cells.