以叶酸受体为靶向的脂质体介导MYCN基因小干扰RNA体外抑制神经母细胞瘤LA-N-5细胞的增殖

目的以氨基脂为主要成分、叶酸受体为靶向的脂质体包裹MYCN基因小干扰性RNA，研究其体外对神经母细胞瘤LA-N-5细胞MYCN基因表达的抑制及细胞增殖的影响。方法制备氨基脂为主要成分、叶酸受体为靶向的脂质体包裹CY3标记的MYCNsiRNA转染LA-N-5细胞，以每微克蛋白中荧光强度半定量siRNA的转染效果；用定量RT-PCR检测转染前后MYCN基因mRNA变化；MTT法检测细胞增殖受抑情况：结果以100nmol／L终浓度转染LA—N-5细胞后每微克蛋白含siRNA（1808．5±140．2）pg，作用72hMYCN基因mRNA表达显著下降，抑制率79．2％，瘤细胞增殖受抑达66．2％。结论叶酸受体为靶向脂质体介导MYCNsiRNA在体外对LA—N-5细胞MYCNmRNA表达有显著抑制作用，明显抑制LA—N-5细胞增殖，为神经母细胞瘤靶向治疗、基因治疗开辟了新的思路。

In vitro inhibition of LA-N-5 cell growth by folate receptor-targeted liposome-induced MYCN gene siRNA inhibits

Objective To study the inhibitory effect of folate receptor-targeted MYCN gene siRNA on the expression and growth of LA-N-5 cells. Methods LA-N-5 cells were transfected with MYCN siRNA marked by folate receptor-targeted liposome-induced Cy3. The transfected siRNA was expressed as fluorescence intensity per μg protein. Changes in MYCN mRNA before and after transfection were detected by real-time quantitative PCR （RT-PCR）. Inhibition of LA-N-5 cell growth was assayed by MTT. Results Perμg protein contained （1 808.5 ± 140.2）pg siRNA in LA-N-5 ceils transfected with 100nmol/L MYCN siRNA. The inhibion rate of MYCN mRNA expression and LA-N-5 cell growth was 79.2% and 66.2%, respectively, 72 hours after transfection. Conclusion Folate receptor-targeted liposome-induced MYCN siRNA can significantly inhibit the expression of MYCN mRNA in LA-N-5 cells and the growth of LA-N-5 cells in vitro,which may open up a new way to the gene and target therapy for neuroblastoma.