| 鞘氨醇激酶基因在3T3-L1脂肪细胞诱导分化中的表达水平 |
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| 引用本文： | 潘晓勤,郭锡熔,丁胜利,龚海霞,倪毓辉,费莉,郭梅,陈荣华. 鞘氨醇激酶基因在3T3-L1脂肪细胞诱导分化中的表达水平[J]. 实用儿科临床杂志, 2003, 18(10): 808-810,T002 |
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| 作者姓名： | 潘晓勤 郭锡熔 丁胜利 龚海霞 倪毓辉 费莉 郭梅 陈荣华 |
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| 作者单位： | 南京医科大学儿科医学研究所,南京210029 |
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| 基金项目： | 国家自然科学基金 (项目编号 :3 9870 3 62 )，江苏省教委自然科学基金(项目编号 :980 0 0 2 ) |
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| 摘 要： | 目的 探讨鞘氨醇激酶基因(SPHK）在3T3-L1脂肪细胞诱导分化中表达水平的变化。方法采用细胞培养和RT-PCR技术检测细胞分化不同阶段脂肪细胞中SPHK基因表达水平。结果1．SPHK基因在3T3-L1脂肪前体细胞诱导分化初期表达呈明显上调趋势；2．随着脂肪前体细胞分化成熟，该基因表达水平明显低于诱导分化初期的基因表达水平。结论 SPHK基因可能参与脂肪细胞分化的调控过程，与肥胖发生有一定联系。
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| 关 键 词： | 鞘氨醇激酶基因 3T3-L1脂肪细胞 细胞分化 表达 肥胖 |
| 文章编号： | 1003-515X(2003)10-0808-03 |
Expression of sphigosine kinase gene in 3T3-L1 preadipocyte differentiation |
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| PAN Xiao-qin,GUO Xi-rong,DING Sheng-li,et al.. Expression of sphigosine kinase gene in 3T3-L1 preadipocyte differentiation[J]. Journal of Applied Clinical Pediatrics, 2003, 18(10): 808-810,T002 |
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| Authors: | PAN Xiao-qin GUO Xi-rong DING Sheng-li et al. |
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| Affiliation: | PAN Xiao-qin,GUO Xi-rong,DING Sheng-li,et al. Institute of Pediatrics Medicine,Nanjing Medical University,Nanjing 210029,P. R. China |
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| Abstract: | Objective To investigate the changes of sphigosine kinase gene expression during 3T3-L1 preadipocyte differentiation. Methods 3T3-L1 preadipocyte was differentiated into the adipocyte. Total RNA from the preadipocytes and adipocytes were extracted and the levels of sphigosine kinase gene mRNA expression were evaluated by RT-PCR.Results In early period of preadipocyte differentiation, the level of sphigosine kinase gene mRNA expression was significantly up-regulated. However, with adipocytes differentiating matured, the level of sphigosine kinase gene mRNA expression was significantly down-regulated, which was significantly lower than that in early period of preadipocyte differentiation. Conclusion Sphigosine kinase gene may be involved in adipocyte differentiation and adipogenesis. |
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| Keywords: | sphigosine kinase gene 3T3-L1 preadipocyte differentiation |
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