| 人急性白血病淋巴细胞HSP70及 HSP70 mRNA的表达研究 |
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| 引用本文： | 吴然,张艳桥,徐文弟.人急性白血病淋巴细胞HSP70及 HSP70 mRNA的表达研究[J].哈尔滨医科大学学报,2001,35(4):259-260,277. |
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| 作者姓名： | 吴然 张艳桥 徐文弟 |
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| 作者单位： | 1. 哈尔滨医科大学生物化学教研室
2. 哈尔滨医科大学附属第二医院内科 |
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| 摘 要： | 目的：检测急性白血病病人血淋巴细胞热休克蛋白70（HSP70）及其mRNA的表达。方法：ELISA法检测HSP70，RT－PCR法检测HSP70 mRNA。结果：化疗前急性白血病病人淋巴细胞HSP70及mRNA明显低于正常人；而化疗后，急性白血病病人淋巴细胞HSP70及mRNA明显高于正常人（P＜0．01），结论：HSP70与急性白血病癌细胞关系密切，对癌细胞起保护作用。
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| 关 键 词： | 热休克蛋白70 急性白血病 ELISA RT－PCR HSP70 HSP70－mRNA |
| 文章编号： | 1000-1905(2001)04-0259-02 |
Research of HSP 70 and HSP70mRNA expression in lymphocyte of human acute leukemia |
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| WU Ran ,ZHANG Yan qiao ,XU Wen di.Research of HSP 70 and HSP70mRNA expression in lymphocyte of human acute leukemia[J].Journal of Harbin Medical University,2001,35(4):259-260,277. |
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| Authors: | WU Ran ZHANG Yan qiao XU Wen di |
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| Institution: | WU Ran 1,ZHANG Yan qiao 2,XU Wen di 1 |
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| Abstract: | Objective To detect the expression of heat shock protein 70 (HSP70) and its mRNA in blood lymphocyte of human acute leukemia. Methods HSP70 was determined by ELISA, HSP70 mRNA was determined by RT-PCR.Results The expression of HSP70 and its mRNA in acute leukemia lymphocyte was much lower than normal before chemotherapy. The expression of HSP70 and its mRNA in acute leukemia lymphocyte was much higher than normal after chemotherapy.Conclusion There is close relationship between acute leukemic cells and HSP70, which protect cancer cells. |
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| Keywords: | Heat Shock Protein 70 acute leukemia ELISA RT-PCR |
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