| 二氢杨梅素对肝癌细胞黏附、侵袭及迁移的抑制作用及机制 |
| |
| 引用本文： | 邱志东,罗芳兰,舒洋,温小军,张庆余,廖雅琳,黎然,缪辉来. 二氢杨梅素对肝癌细胞黏附、侵袭及迁移的抑制作用及机制[J]. 中国普通外科杂志, 2015, 24(9): 1263-1268 |
| |
| 作者姓名： | 邱志东 罗芳兰 舒洋 温小军 张庆余 廖雅琳 黎然 缪辉来 |
| |
| 作者单位： | （1. 广东省深圳市第七人民医院 普通外科，广东 深圳 518000；2. 广东医学院研究生学院，广东 湛江 524000；3. 广东医学院附属医院 肝胆外科，广东 湛江524000；4. 广东医学院第一临床学院，广东 湛江 524000） |
| |
| 摘 要： | 目的：研究二氢杨梅素（DHM）对肝癌细胞黏附、侵袭和迁移能力的影响及其可能机制。方法：用不同浓度DHM处理肝癌MHCC97L细胞后，分别检测细胞的黏附能力、迁移与侵袭能力，以及E-cadherin、MMP-2、MMP-9和VEGF蛋白表达。结果：与空白对照细胞比较，DHM处理后的MHCC97L细胞黏附力明显降低、侵袭与迁移力明显减弱（均P<0.05）；E-cadherin表达明显上调，而MMP-9、VEGF蛋白表达明显下调的水平（均P<0.05），但MMP-2蛋白的表达无明显改变（均P>0.05）。结论：DHM可能通过调控E-cadherin、MMP-9和VEGF蛋白的表达抑制肝癌细胞的黏附、迁移和侵袭。
|
| 关 键 词： | 癌，肝细胞；二氢杨梅素；肿瘤侵润 |
| 收稿时间： | 2015-01-14 |
| 修稿时间： | 2015-08-05 |
Inhibitory effect of dihydromyricetin on adhesion, invasion and migration in hepatocellular carcinoma cells and the mechanism |
| |
| QIU Zhidong,LUO Fanglan,SHU Yang,WEN Xiaojun,ZHANG Qingyu,LIAO Yalin,LI Ran,MIAO Huilai. Inhibitory effect of dihydromyricetin on adhesion, invasion and migration in hepatocellular carcinoma cells and the mechanism[J]. Chinese Journal of General Surgery, 2015, 24(9): 1263-1268 |
| |
| Authors: | QIU Zhidong LUO Fanglan SHU Yang WEN Xiaojun ZHANG Qingyu LIAO Yalin LI Ran MIAO Huilai |
| |
| Affiliation: | (1. Department of General Surgery, the Seventh People''s Hospital of Shenzhen, Shenzhen 518000, China; 2. Graduate School, Guangdong Medical College, Zhanjiang, Guangdong 524000, China; 3. Department of Hepatobiliary Surgery, Affiliated Hospital, Guangdong Medical College, Zhanjiang, Guangdong 524000, China; 4. The First Clinical College of Guangdong Medical College, Zhanjiang, Guangdong 524000, China) |
| |
| Abstract: | Objective: To investigate the effect of dihydromyricetin (DHM) on adhesion, invasion and migration abilities of hepatocellular carcinoma (HCC) cells and the possible mechanisms.Methods: HCC MHCC97L cells were exposed to different concentrations of DHM, and then, the adhesion, invasion and migration abilities of the cells were examined, and the protein expressions of E-cadherin, MMP-2, MMP-9 and VEGF were also determined.Results: In MHCC97L cells treated with DHM compared with blank control cells, the abilities of adhesion, invasion and migration were all significantly deceased (all P<0.05), the E-cadherin expression was upregulated, and the MMP-9 and VEGF expressions were downregulated significantly, but the MMP-2 expression showed no significant change (P>0.05). Conclusion: DHM can inhibit the adhesion, invasion and migration of HCC cells by regulation of E-cadherin, MMP-9 and VEGF expressions. |
| |
| Keywords: | Carcinoma, Hepatocellular Dihydromyricetin Neoplasm Invasiveness |
|
| 点击此处可从《中国普通外科杂志》浏览原始摘要信息 |
|
点击此处可从《中国普通外科杂志》下载免费的PDF全文 |
|
