CTHRC1在胰腺癌中的表达及其意义

目的:观察胶原三股螺旋重复蛋白1(CTHRC1)在胰腺癌组织中的表达及其对胰腺癌细胞生物学行为的影响。方法:采用实时定量PCR(RT-PCR)方法检测40例胰腺患者癌组织与癌旁组织中CTHRC1的表达。胰腺癌Panc28细胞转染pcDNA3.1-CTHRC1或CTHRC1 siRNA后,以未转染的Panc28细胞为对照,采用克隆形成实验检测CTHRC1对胰腺癌Panc28细胞增殖,伤口愈合实验和Boyden小室检测细胞迁移和侵袭能力。结果:RT-PCR结果显示,胰腺癌组织CTHRC1 mRNA表达水平明显高于癌旁组织(P0.05)。与对照组Panc28细胞比较,转染pcDNA3.1-CTHRC1上调CTHRC1后,Panc28细胞克隆形成数明显增加、伤口宽度百分比明显减少、侵袭的细胞数明显增多,而转染CTHRC1 siRNA下调CTHRC1表达后,Panc28细胞以上指标呈反向变化,差异均有统计学意义(均P0.05)。结论:胰腺癌组织在CTHRC1表达升高,CTHRC1可能通过促进胰腺癌细胞的增殖、迁移和侵袭能力而参与胰腺癌的发生与发展。

CTHRC1 expression in pancreatic cancer and its significance

Objective: To investigate the expression of collagen triple helix repeat containing 1 (CTHRC1) in pancreatic cancer tissue, and its influence on biobehavior of pancreatic cancer cells.Methods: The expression of CTHRC1 mRNA in the specimens of cancer and adjacent tissue from 40 pancreatic cancer patients was detected by real time PCR (RT-PCR). Pancreatic cancer Panc28 cells were transfected with pcDNA3.1-CTHRC1 or CTHRC1 siRNA using untransfected Panc28 cells as control, and then the cell proliferation, migration and invasion ability were determined by colony formation assay, wound healing assay and Boyden chamber assay, respectively.Results: Results of the RT-PCR showed that CTHRC1 expression level in pancreatic cancer tissue was significantly higher than that in adjacent tissue (P<0.05). Compared with control Panc28 cells, the number of colony formation was increased, percentage of wound width was reduced and number of invaded cells was increased significantly in Panc28 cells with up-regulated CTHRC1 expression after pcDNA3.1-CTHRC1 transfection, while the opposite changes were seen in those with down-regulated CTHRC1 expression after CTHRC1 siRNA transfection, and all the differences had statistical significance (all P<0.05).Conclusion: CTHRC1 expression is increased in pancreatic cancer tissue, which may contribute to the occurrence and development of pancreatic cancer through promoting cell proliferation, and migration and invasion ability of the pancreatic cancer cells.