域含蛋白7A在肝细胞癌中的表达及其与预后的关系

目的:探讨域含蛋白7A(THSD7A)基因在肝细胞癌(HCC)中的表达情况及临床意义。方法:分别用RT-PCR与免疫组化法检测30例新鲜HCC及癌旁组织标本中THSD7A m RNA表达及75例HCC及癌旁组织石蜡标本中THSD7A蛋白的表达,分析其与HCC患者临床病理因素及预后的关系。结果:与癌旁组织比较,在HCC组织中THSD7A m RNA表达下调(P0.05);THSD7A在HCC组织中的阳性表达率明显低于癌旁组织(24.0%vs.87.0%,P0.05)。THSD7A蛋白的表达与患者结节数目(P=0.011)、Edmondson-Steiner分级(P=0.013)、BCLC分期(P=0.048)有关。THSD7A蛋白低表达患者总体生存率(P=0.016)与无瘤生存率(P=0.013)均明显低于THSD7A高表达患者。结论:THSD7A在HCC中可能发挥抑癌基因的作用,THSD7A表达降低患者预后不良。

Expression of domain containing 7A in hepatocellular carcinoma and its prognostic significance

Objective: To investigate the domain containing 7A (THSD7A) expression in human hepatocellular carcinoma (HCC) and its clinical significance. Methods: The THSD7A mRNA expression in 30 fresh specimens of HCC with paired and adjacent tissues, and protein expression in 75 paraffin embedded specimens of HCC with paired adjacent tissues were detected by RT-PCR and immunohistochemical staining, respectively. The relations of THSD7A protein expression with clinicopathologic parameters and prognosis of HCC patients were analyzed. Results: The THSD7A mRNA expression in HCC tissue was significantly down-regulated compared with adjacent tissue (P<0.05), and the positive expression rate of THSD7A protein in HCC tissue was significantly lower than that in adjacent tissue (24.0% vs. 87.0%, P<0.05). The THSD7A protein expression was significantly related to the tumor numbers (P=0.011), Edmondson-Steiner grade (P=0.013) and BCLC stage (P=0.048) of the patients. Both overall survival and disease-free survival in patients with low THSD7A expression were significantly lower than those in patients with high THSD7A expression (P=0.016, P=0.013). Conclusion: THSD7A may play a tumor-suppressor role in HCC, and those with decreased THSD7A expression have a relatively poor prognosis.