Multiple column peptide synthesis,Part 2 (1, 2) |
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Authors: | MORTEN MELDAL CHARLOTTE BISGAARD HOLM GUSTAV BOJESEN MOGENS HAVSTEEN JAKOBSEN ARNE HOLM |
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Abstract: | A manually operated apparatus for parallel multiple column soli-phase peptide synthesis is described. It employs Fmoc-amino acid-O-Dhbt or -Pfp esters in the continuous flow version of the polyamide method on small packed columns of kieselguhr supported resin in a reaction block of Teflon. The solvents and deprotecting reagents are dispensed from two washers in a parallel fashion and reagent consumption is low. Activated and protected amino acids are transferred from a dispenser tray as solutions, eight at a time. The use of the method is demonstrated by the synthesis of overlapping peptides from a protein structure and of analogous protease substrates. The products have been characterized by HPLC, FAB mass spectroscopy and amino acid analysis. |
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Keywords: | bordetella pertussis Dhbt-esters Pfp-esters parallel multiple column peptide synthesis protease substrate |
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