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Multiple column peptide synthesis,Part 2 (1, 2)
Authors:MORTEN MELDAL  CHARLOTTE BISGAARD HOLM  GUSTAV BOJESEN  MOGENS HAVSTEEN JAKOBSEN  ARNE HOLM
Abstract:A manually operated apparatus for parallel multiple column soli-phase peptide synthesis is described. It employs Fmoc-amino acid-O-Dhbt or -Pfp esters in the continuous flow version of the polyamide method on small packed columns of kieselguhr supported resin in a reaction block of Teflon. The solvents and deprotecting reagents are dispensed from two washers in a parallel fashion and reagent consumption is low. Activated and protected amino acids are transferred from a dispenser tray as solutions, eight at a time. The use of the method is demonstrated by the synthesis of overlapping peptides from a protein structure and of analogous protease substrates. The products have been characterized by HPLC, FAB mass spectroscopy and amino acid analysis.
Keywords:bordetella pertussis  Dhbt-esters  Pfp-esters  parallel multiple column peptide synthesis  protease substrate
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