| mTOR信号通路调控CD133阳性胶质瘤干细胞自我更新的分子机制 |
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| 引用本文： | 付军,刘晓梅,陈忠平.mTOR信号通路调控CD133阳性胶质瘤干细胞自我更新的分子机制[J].中国神经肿瘤杂志,2010(2):75-81. |
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| 作者姓名： | 付军 刘晓梅 陈忠平 |
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| 作者单位： | 华南肿瘤学国家重点实验室;中山大学肿瘤防治中心神经外科/神经肿瘤科; |
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| 基金项目： | 国家自然科学基金(No.30772551) |
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| 摘 要： | 背景与目的：mTOR（mammalian target of rapamycin）信号通路异常活化和高级别胶质瘤患者的预后不良相关。本研究探讨mTOR信号通路调控胶质瘤干细胞（GSCs）自我更新相关的分子机制。方法：采用CD133免疫磁珠分选CD133阳性胶质瘤干细胞。Western blot检测mTOR信号通路组分p-S6K、p-S6和干细胞自我更新相关基因Bmi-1的表达水平;Rapamycin（RPA）阻断mTOR信号通路后,用肿瘤球形成实验评价干预mTOR信号通路对胶质瘤干细胞自我更新的影响;统计学处理采用SPSS11.0统计分析软件分析。结果：CD133阳性胶质瘤干细胞表达较高水平的mTOR信号通路组分p-S6K、p-S6和干细胞自我更新相关分子Bmi-1。通过rapamycin阻断mTOR信号通路,可诱导胶质瘤干细胞谱系分化,同时显著降低肿瘤球形成能力（P〈0.05）,而自噬抑制剂3-MA处理不能逆转rapamycin的效应。结论：mTOR信号通路能够调控胶质瘤干细胞自我更新,阻断mTOR通路下调胶质瘤干细胞自我更新潜能。
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| 关 键 词： | 胶质瘤干细胞 mTOR信号通路 自我更新 |
mTOR Signaling Pathway Regulates Self-renewal of CD133 Positive Glioma Stem Cells |
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| Jun Fu,Xiao-mei Liu,Zhong-ping Chen, .State Key Laboratory of Oncology in South China,Guangzhou ,P.R.China.mTOR Signaling Pathway Regulates Self-renewal of CD133 Positive Glioma Stem Cells[J].Chinese Journal of Neuro-Oncology,2010(2):75-81. |
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| Authors: | Jun Fu Xiao-mei Liu Zhong-ping Chen State Key Laboratory of Oncology in South China Guangzhou PRChina |
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| Institution: | Jun Fu1,2,Xiao-mei Liu1,Zhong-ping Chen1,2 1.State Key Laboratory of Oncology in South China,Guangzhou 510060,P.R.China,2.Department of Neurosurgery/Neuro-oncology,Cancer Center,Sun Yat-sen University |
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| Abstract: | BACKGROUND & OBJECTIVE:Activation of mammalian target of rapamycin(mTOR)has been associated to poor prognosis of glioma patients.This study was designed to investigate the regulatory mechanisms of mTOR signaling on self-renewal of glioma stem cells(GSCs).METHODS:CD133+GSCs have been isolated by using CD133 magnetic beads from human glioblastoma specimens and glioma cell line SKMG-4 and were propagated without serum in vitro.Molecular markers for glioma stem cells(CD133,nestin,Sox-2,GFAP and TuJ1) were detec... |
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| Keywords: | Glioma stem cells mTOR Selfrenewal |
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