西罗莫司洗脱支架预防再狭窄量效关系的实验研究

目的观察不同剂量的国产西罗莫司洗脱支架预防再狭窄的有效性。方法广西种小型猪18只，分别植入不同剂量西罗莫司洗脱支架，常规剂量组：为有效的西罗莫司涂层常规剂量140μg／cm^2；0．75剂量组和0．5剂量组：分别含西罗莫司常规剂量的75％（105μg／cm^2）和50％（70μg／cm^2）。在术后28d和90d，用组织病理检查冠状动脉内膜增生程度，免疫组化检测内膜平滑肌细胞中增殖细胞核抗原和TUNEL法检测凋亡细胞。结果支架部位血管腔面积以常规剂量组最大。0．75剂量组次之，而0．5剂量组最小。支架植入后3组血管新生内膜均可见增殖细胞核抗原阳性细胞。以常规剂量组最少。凋亡细胞仅见于常规剂量组和常规剂量组新生内膜。观察至90d，3组动物均未见血栓。结论预防再狭窄以常规剂量组的药物洗脱支架效果最好，有抑制内膜过度增生的作用，0．75剂量组达到同常规剂量组相似的预防冉狭窄效果。

Preclinical study of the dose-effect relationship of sirolimus-eluting stent on coronary restenosis prevention

Objectives To evaluate the dose-effect relationship of sirolimus-eluting stent on coronary restenosis prevention in a mini-swine model. Methods Eighteen mini-swines were randomized to 3 drug dose groups. Sirolimus-eluting stent was coated with 3 different drug doses, including 50%,75% and 100%. Arteries were examined by histopathology,histomorphometry and immunohistochemistry methods in 28 days and 90 days later.Results The 28th and 90th day following results showed that the luminal area of 75% dose group w...