Synthesis and purification of [2‐13C]‐5‐fluorouracil |
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| Authors: | Huzaifa S Rangwala John W Giraldes Vadim J Gurvich |
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| Institution: | 1. Institute for Therapeutics Discovery and Development, College of Pharmacy, University of Minnesota, Minneapolis, MN, USA;2. Department of Medicinal Chemistry, College of Pharmacy, University of Minnesota, Minneapolis, MN, USA |
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| Abstract: | 2‐13C]‐5‐Fluoropyrimidine‐2,4(1H,3H)‐dione (2‐13C]‐5‐fluorouracil or 2‐13C]‐5‐FU) is a potential diagnostic agent for measuring 5‐FU‐induced toxicity in cancer patients. It was prepared and purified with isotopic and chemical purity of>99% on a multigram scale in a two‐step synthesis from 13C]‐urea. Preparative separation of 2‐13C]‐FU and 2‐13C]‐uracil was carried out by automated medium pressure silica gel column chromatography. The method is applicable to a broader range of 5‐FU isotopic analogs derived from labeled uracil. Copyright © 2011 John Wiley & Sons, Ltd. |
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| Keywords: | 5‐FU [2‐13C]‐5‐fluorouracil carbon‐13 stable isotope 5‐FU/uracil separation |
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