利用限制性片段长度多态性技术鉴别梅花鹿骨种属来源的研究

摘 要 目的：建立一种基于限制性片段长度多态性技术鉴别梅花鹿骨粉的种属来源的方法。方法: 对脱钙后的动物骨粉样品进行核糖核酸提取，通过聚合酶链式反应对梅花鹿特征片段进行扩增，并通过限制性片段长度多态性分析进一步对其种属来源进行确证。结果: 通过对特征片段进行聚合酶链式反应扩增，可将梅花鹿及马鹿来源的骨粉样品与牛、猪、狗等动物骨粉样品进行区分。通过对限制性内切酶XbaI进行酶切后的片段长度进行分析，可进一步区分梅花鹿与马鹿来源的骨粉样品。结论:建立了一种基于限制性片段长度多态性技术的梅花鹿骨粉种属来源的鉴别方法。

Origin Determination of Sika Deer Bones by Restriction Fragment Length Polymorphism

ABSTRACT Objective: To establish a method of restriction fragment length polymorphism (RFLP) to determine the origin of sika deer bones. Methods: The DNA in the bone samples was extracted after decalcification, and then amplified using polymerase chain reaction (PCR). The origin of the samples was further identified using RFLP analysis. Results:The bone samples of sika deer and red deer could be distinguished from those of pig, bovine and dog by PCR. And the samples of sika deer and red deer could be further distinguished by RFLP through the analysis of the length of restriction enzyme XbaI. Conclusion:A RFLP method is established to determine the origin of sika deer bones.