| 消癌解毒方含药血清对人结肠癌细胞增殖及糖酵解过程的影响 |
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| 引用本文： | 石文静,谭佳妮,沈卫星,徐长亮,孙东东,程海波.消癌解毒方含药血清对人结肠癌细胞增殖及糖酵解过程的影响[J].中国实验方剂学杂志,2017,23(20):120-125. |
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| 作者姓名： | 石文静 谭佳妮 沈卫星 徐长亮 孙东东 程海波 |
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| 作者单位： | 南京中医药大学 转化医学研究中心, 国家中医药管理局名医验方评价与转化重点研究室, 江苏省抗肿瘤验方与产业化工程实验室, 南京 210023;南京中医药大学 第一临床医学院, 南京 210023,南京中医药大学 转化医学研究中心, 国家中医药管理局名医验方评价与转化重点研究室, 江苏省抗肿瘤验方与产业化工程实验室, 南京 210023,南京中医药大学 转化医学研究中心, 国家中医药管理局名医验方评价与转化重点研究室, 江苏省抗肿瘤验方与产业化工程实验室, 南京 210023;江苏省中医药防治肿瘤协同创新中心, 南京 210023,南京中医药大学 转化医学研究中心, 国家中医药管理局名医验方评价与转化重点研究室, 江苏省抗肿瘤验方与产业化工程实验室, 南京 210023,南京中医药大学 转化医学研究中心, 国家中医药管理局名医验方评价与转化重点研究室, 江苏省抗肿瘤验方与产业化工程实验室, 南京 210023;江苏省中医药防治肿瘤协同创新中心, 南京 210023,南京中医药大学 转化医学研究中心, 国家中医药管理局名医验方评价与转化重点研究室, 江苏省抗肿瘤验方与产业化工程实验室, 南京 210023;江苏省中医药防治肿瘤协同创新中心, 南京 210023 |
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| 基金项目： | 国家自然科学基金项目（81373511，81573910，81673559，81403079）；江苏省自然科学基金项目（BK20141467，BK20161045）；江苏省高校自然科学基金项目（16KJB360001）；江苏高校优势学科建设工程二期项目 |
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| 摘 要： | 目的:探讨不同浓度消癌解毒方含药血清对人结肠癌细胞增殖及糖酵解过程的影响及其作用机制。方法:8只新西兰兔随机分为含药血清组(消癌解毒方40 g·kg·~(-1)d~(-1))和空白血清组(等体积生理盐水),每组兔子连续灌胃3 d,制备消癌解毒方含药血清和空白血清。5%,10%,15%消癌解毒方含药血清处理人结肠癌HT-29与HCT-116细胞后,采用噻唑蓝比色法测定其对HT-29与HCT-116细胞生长的抑制作用,乳酸试剂盒检测HT-29与HCT-116细胞的乳酸生成量,葡萄糖试剂盒检测HT-29与HCT-116细胞的葡萄糖生成量,蛋白免疫印迹法(Western blot)检测己糖激酶(HK2),丙酮酸脱氢酶激酶(PDK1),乳酸脱氢酶(LDHA)和低氧诱导因子-1α(HIF-1α)蛋白表达。结果:消癌解毒方含药血清能够抑制HT-29与HCT-116细胞增殖能力,15%消癌解毒方含药血清对人结肠癌HT-29与HCT-116细胞的抑制率分别为42.7%,50.2%。Western blot结果显示,与空白组比较,消癌解毒方含药血清可降低HK2,PDK1,LDHA,HIF-1α蛋白相对表达量(P0.05),且随浓度的增加表达降低。乳酸及葡萄糖试剂盒检测结果显示,与空白组比较,消癌解毒方含药血清可降低HT-29与HCT-116细胞的乳酸及葡萄糖水平(P0.05),且随浓度的增加乳酸及葡萄糖水平降低。结论:消癌解毒方含药血清可以通过抑制人结肠癌HT-29与HCT-116细胞糖酵解的过程从而抑制肿瘤细胞的增殖,其抑制糖酵解过程的机制可能与降低HIF-1α表达,进而减少糖酵解相关酶HK2,PDK1,LDHA表达有关。
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| 关 键 词： | 消癌解毒方 结肠癌 增殖 糖酵解 |
| 收稿时间： | 2017/2/9 0:00:00 |
Effect of Serum Containing Xiaoai Jiedu Decoction on Proliferation and Glycolysis of Human Colon Cancer Cells |
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| SHI Wen-jing,TAN Jia-ni,SHEN Wei-xing,XU Chang-liang,SUN Dong-dong and CHENG Hai-bo.Effect of Serum Containing Xiaoai Jiedu Decoction on Proliferation and Glycolysis of Human Colon Cancer Cells[J].China Journal of Experimental Traditional Medical Formulae,2017,23(20):120-125. |
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| Authors: | SHI Wen-jing TAN Jia-ni SHEN Wei-xing XU Chang-liang SUN Dong-dong and CHENG Hai-bo |
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| Institution: | Translational Medicine Research Center, Nanjing University of Chinese Medicine, Key Laboratory of Famous Doctors''Proved Recipe Evaluation and Transformation of State Administration of Traditional Chinese Medicine(TCM), Provincial Laboratory of Anticarcinoma Proved Recipe Research and Engineering Industrialization, Nanjing 210023, China;The First Clinical Medical College of Nanjing University of Chinese Medicine, Nanjing 210023, China,Translational Medicine Research Center, Nanjing University of Chinese Medicine, Key Laboratory of Famous Doctors''Proved Recipe Evaluation and Transformation of State Administration of Traditional Chinese Medicine(TCM), Provincial Laboratory of Anticarcinoma Proved Recipe Research and Engineering Industrialization, Nanjing 210023, China,Translational Medicine Research Center, Nanjing University of Chinese Medicine, Key Laboratory of Famous Doctors''Proved Recipe Evaluation and Transformation of State Administration of Traditional Chinese Medicine(TCM), Provincial Laboratory of Anticarcinoma Proved Recipe Research and Engineering Industrialization, Nanjing 210023, China;TCM Treatment on Tumor Collaborative Innovation Center of Jiangsu Provine, Nanjing 210023, China,Translational Medicine Research Center, Nanjing University of Chinese Medicine, Key Laboratory of Famous Doctors''Proved Recipe Evaluation and Transformation of State Administration of Traditional Chinese Medicine(TCM), Provincial Laboratory of Anticarcinoma Proved Recipe Research and Engineering Industrialization, Nanjing 210023, China,Translational Medicine Research Center, Nanjing University of Chinese Medicine, Key Laboratory of Famous Doctors''Proved Recipe Evaluation and Transformation of State Administration of Traditional Chinese Medicine(TCM), Provincial Laboratory of Anticarcinoma Proved Recipe Research and Engineering Industrialization, Nanjing 210023, China;TCM Treatment on Tumor Collaborative Innovation Center of Jiangsu Provine, Nanjing 210023, China and Translational Medicine Research Center, Nanjing University of Chinese Medicine, Key Laboratory of Famous Doctors''Proved Recipe Evaluation and Transformation of State Administration of Traditional Chinese Medicine(TCM), Provincial Laboratory of Anticarcinoma Proved Recipe Research and Engineering Industrialization, Nanjing 210023, China;TCM Treatment on Tumor Collaborative Innovation Center of Jiangsu Provine, Nanjing 210023, China |
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| Abstract: | |
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| Keywords: | Xiaoai Jiedu decoction colon cancer proliferation glycolysis |
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