共表达HIV-1 gag-gp120与IL-6重组鸡痘病毒和核酸疫苗的联合免疫研究

目的探讨共表达HIV-1 gag-gpl20与IL-6重组鸡痘病毒和核酸疫苗联合免疫小鼠的免疫应答。方法以重组鸡痘病毒首免，以表达相同抗原的核酸疫苗质粒追加免疫，检测免疫小鼠脾特异性CTL杀伤活性和血清抗体水平。结果联合免疫组脾特异性CTL杀伤活性比重组鸡痘病毒单独免疫组、核酸疫苗单独免疫组、鸡痘病毒疫苗株对照组和空白质粒对照组高，血清抗体水平显著高于空白质粒对照组和鸡痘病毒疫苗株对照组，但与重组鸡痘病毒单独免疫组、核酸疫苗单独免疫组之间差异无显著性。结论重组鸡痘病毒和核酸疫苗的联合免疫可诱导小鼠产生更强的细胞免疫应答。

The immune response in mice induced by co-inoculating recombinant fowlpox virus coexpressing HIV-1 gag-gp120 and IL-6 and nucleic acid vaccine

Objective To explore the immune response in mice induced by co inoculation of coexpressing HIV 1 gag gp120 and IL 6 recombinant fowlpox virus (rFPV) and nucleic acid vaccine. Methods BALB/c mice were immunized with rFPV and boosting with nucleic acid vaccine expressing the same antigen. The specific killing activities of spleen CTL and the levels of serum antibodies from the immunized mice were examined. Results The specific killing activities of spleen CTL of the combined immunization group were higher than those of rFPV group, nucleic acid vaccine group, FPV vaccine strain control group and the vector plasmid control group. The titers of serum antibodies were also significantly higher than those of FPV vaccine strain control group and the vector plasmid control group, but there was no significant differences between the combined immunization group and the single immunization groups. Conclusion The combined immunization with rFPV and nucleic acid vaccine elicited higher cellular immunity in mice.