VEGF反义寡核苷酸抑制胆囊癌生长增殖和凋亡的体外实验研究

目的:探讨VEGF反义寡核苷酸转染对人胆囊癌GBC-SD细胞生长、增殖和凋亡的影响。方法:运用Oligofectamine介导VEGF反义寡核苷酸(antisense oligodeoxynucleotides,ASODN)和错义寡核苷酸(Scrambled Oligodeoxynucleotide,SODN)转染人胆囊癌(GBC-SD)细胞。采用MTT法测定转染后各组细胞的生长曲线及抑制率,流式细胞术检测转染后不同时间各组细胞凋亡情况。结果:SODN组、SODN十Oligofectamine组对GBC-SD细胞生长无显著影响(P>0.05),ASODN组及ASODN十Oligofectamine组则能显著抑制GBC-SD细胞生长(P<0.05),且ASODN十Oligofectamine组对GBC-SD细胞生长增殖抑制作用较ASODN组更为强(P<0.05)。流式细胞术检测结果发现ASODN十Oligofectamine组能促进GBC-SD细胞凋亡(P<0.05)。结论:VEGFASODN转染能抑制胆囊癌GBC-SD细胞生长和增殖,Oligofectamine介导能明显增强VEGF ASODN的抑制作用;Oligofectamine介导VEGF反义寡核苷酸转染能促进胆囊癌GBC-SD细胞的凋亡。

The effect of oligofectamine mediated VEGF ASODN transfection on GBC-SD cell growth,proliferation and apoptosis in vitro

Objective: To investigate the effect of oligofectamine mediated VEGF ASODN transfection on GBC-SD cell growth,proliferation and apoptosis in vitro.Methods: GBC-SD cells were transfected VEGF ASODN and SODN mediated by oligofectamine.The transfected GBC-SD cells of each groups were assessed the change of growth and proliferation activity by MTT,and apoptosis by flow cytometry.Results: The transfected GBC-SD cells growth and proliferation activity tested by MTT in groups of SODN and SODN oligofectamine had not any difference as compared with control group(P>0.05),but as for the cells in groups of ASODN and ASODN oligofectamine,the growth and proliferation activity were significantly inhibited(P<0.05),moreover,the effect of ASODN oligofectamine was more significant than that of ASODN(P<0.05).The results of flow cytometry revealed ASODN oligofectamine could accelerate transfected GBC-SD cells apoptosis(P<0.05).Conclusion: VEGF ASODN could inhibit GBC-SD cells growth,proliferation and accelerate apoptosis.The oligofectamine could strengthen the effect of ASODN.