| 麻花秦艽组织培养及植株再生的研究 |
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| 引用本文： | 刘丽莎,张西玲,王岚,张延红.麻花秦艽组织培养及植株再生的研究[J].中国中药杂志,2006,31(10):797-800. |
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| 作者姓名： | 刘丽莎 张西玲 王岚 张延红 |
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| 作者单位： | 甘肃中医学院,甘肃,兰州,730000 |
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| 摘 要： | 目的：研究药用植物麻花秦艽组织培养技术,为工厂化育苗提供科学依据。方法：以麻花秦艽根、茎育出的无菌苗为外植体,采用MS培养基,附加不同的植物激素进行实验。结果：以MS培养基为基本培养基,附加BA 0.5～1.0 mg·L-1+NAA 0.5 mg·L-1,适于丛生芽的诱导与增殖；附加IAA 1.0 mg·L-1+BA 3 mg·L-1,适于愈伤组织的诱导；附加IAA 1.0 mg·L-1+BA 2.0～3.0 mg·L-1适于愈伤组织继代培养,附加BA 2.0 mg·L-1+NAA 0.5 mg·L-1适于愈伤组织的分化。结论：通过诱导愈伤组织途径可以达到快速繁殖的目的。
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| 关 键 词： | 麻花秦艽 组织培养 植株再生 |
| 文章编号： | 1001-5302（2006）10-0797-04 |
| 收稿时间： | 2/1/2004 12:00:00 AM |
| 修稿时间： | 2004-02-01 |
Studies on plantlet regeneration and propagation of Gentiana stramines |
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| LIU Li-sha;ZHANG Xi-ling;WANG Lan;ZHANG Yan-hong.Studies on plantlet regeneration and propagation of Gentiana stramines[J].China Journal of Chinese Materia Medica,2006,31(10):797-800. |
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| Authors: | LIU Li-sha;ZHANG Xi-ling;WANG Lan;ZHANG Yan-hong |
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| Institution: | Gansu College of Traditional Chinese Medicine, Lanzhou 730000, China. |
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| Abstract: | Objective:To provide scientific basis for large scale production by studying the technique of tissue culture ofGentiana stramines.Method:Callus was induced from germ-free stem segment ofG.stramineson a MS medium supplemented with different hormones.Result:The MS medium with 0.5-1.0 mg.L-1BA and 0.5 mg.L-1NAA was suitable for the induction and proliferation of cluster bads.MS medium with 1.0 mg.L-1IAA and 3 mg.L-1BA was suitable for the induction of calli.MS medium with 1.0 mg.L-1IAA and 2.0-3.0 mg.L-1BA was suitable for the subculture of calli.MS medium with 2.0 mg.L-1BA and 0.5 mg.L-1NAA was suitable for the differentiation of calli.Conclution:Aseptic seeding ofG.straminescan be quickly propagated by shoot culture. |
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| Keywords: | Gentiana stramines tissue culture plant regeneration |
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