| TiO2喷砂酸蚀处理对人成骨细胞骨保护素和骨保护素配体mRNA表达影响的研究 |
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| 引用本文： | 陆斌,李广琪,李建武,谢延,丁宏,苏杭,杨艳.TiO2喷砂酸蚀处理对人成骨细胞骨保护素和骨保护素配体mRNA表达影响的研究[J].中国矫形外科杂志,2011,19(3):238-240. |
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| 作者姓名： | 陆斌 李广琪 李建武 谢延 丁宏 苏杭 杨艳 |
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| 作者单位： | 武警陕西省总队医院骨科,西安市,710054 |
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| 摘 要： | 目的]探讨纯钛钛片经过喷砂及喷砂酸蚀处理后对人成骨细胞系MG63细胞骨保护素(osteoprotegerin,OPG)及骨保护素配体(osteoprotegerin ligand,OPGL)mRNA表达水平的影响.方法]纯钛钛片表面分别进行机械打磨、喷砂及喷砂酸蚀处理,将人成骨细胞系MG63细胞接种于钛片表面,采用荧光实时定量PCR法检测OPG、OPGL mRNA表达水平.结果]MG63细胞在经过喷砂及喷砂酸蚀处理后的钛片上培养后其OPG mRNA水平增高,与机械打磨组相比有统计学意义(P＜0.05),而OPGL mRNA表达水平在各组之间没有明显差异(P＞0.05).结论]经过喷砂及喷砂酸蚀处理的钛片均可促进人成骨细胞表达OPG,从而调节成骨细胞与破骨细胞之间的平衡,促进骨质重建.
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| 关 键 词： | 喷砂酸蚀处理 成骨细胞 骨保护素 骨保护素配体 |
Effects of TiO_2 sandblast and acid-etched titanium surfaces on mRNA expression of osteoprotegerin and osteoprotegerin ligand in human osteoblast |
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| LU Bin,LI Guang-qi,LI Jian-wu,et al..Effects of TiO_2 sandblast and acid-etched titanium surfaces on mRNA expression of osteoprotegerin and osteoprotegerin ligand in human osteoblast[J].The Orthopedic Journal of China,2011,19(3):238-240. |
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| Authors: | LU Bin LI Guang-qi LI Jian-wu |
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| Institution: | LU Bin,LI Guang-qi,LI Jian-wu,et al.Department of Orthopaedics,Chinese People's Armed Police Troops Shanxi Province Corps Hospital,Xi'an 710054,China |
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| Abstract: | Objective]To investigate the effectiveness of TiO2 sandblasted and acid-etched surfaces of titanium on mRNA expression of osteoprotegerin and osteoprotegerin ligand in human MG63 cells. Methods]Titanium discs were prepared by machine-polished,sandblasted and acid-etched.MG63 cells were cultured on the titanium surface.The mRNA expressions of OPG and OPGL in MG63 cells were analyzed by real-time PCR.Results]The level of OPG mRNA was increased in sandblasted group and sandblasted and acid-etched group.Ther... |
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| Keywords: | sandblasted and acid-etched osteoblast osteoprotegerin osteoprotegerin ligand |
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