细茎石斛不同部位有效成分及分布规律研究

目的 :探索多糖、生物碱、微量元素等有效成分在细茎石斛不同部位中的含量分布情况以及加工处理工艺对石斛质量的影响。方法 :分析细茎石斛茎的上、中、下部及根部中的多糖、生物碱、必需微量元素含量。应用硫酸 苯酚法测定多糖 ;氯仿 溴甲酚绿法测定总生物碱 ;ICP等离子体发射光谱法测定微量元素。结果 :细茎石斛含有较高的多糖、总生物碱和钾、钙、镁、铁、锰、锶、钛、铜等微量元素 ,不同部位含量存在较为显著差异 ;干燥工艺对细茎石斛多糖含量及不同部位的多糖、生物碱分布有较大影响。结论 :细茎石斛含有较高的多糖、生物碱、微量元素等活性物质 ;石斛干燥加工以高温杀青后 60℃烘干为宜。

Studies on Polysaccharide Alkaloids and Minerals from Dendrobium moniliforme (L.)Sw.

Objective: To explore contents of active substances in different part of Dendrobium monilifrome and the quality influenced by different drying processes. Method: Contents of alkaloids and polysaccharides in upper, middle, lower-stem and root part of Dendrobium moniliforme were determined by uv-spectrophotometer method. Contents of essential trace elements were determined by ICP method. Result: Contents of polysaccharides and alkaloids were highest in the upper-stem of D. montiliforme. Contents of active substances in different part of D. moniliforme were unequal. Distributions of polysaccharides and alkaloids in different parts were greatly influenced by drying procedures. Conclusion: Dendrobium moniliforme has higher contents of active ingredients, such as essential tract elements, alkaloids and polysaccharides. The ideal drying process after harvesting is to be the way dried by fire at a high temperature and desiccated at a temperature of 60 degreeC.