针刺对哮喘模型大鼠支气管肺泡灌洗液中炎性细胞的影响

目的:探讨针刺对哮喘模型大鼠支气管肺泡灌洗液(BALF)中白细胞及嗜酸粒细胞(EOS)的影响.方法:将30只清洁级雄性SD大鼠,随机分为空白组、模型组和针刺组,每组10只.针刺组和模型组采用腹腔注射卵蛋白(OVA)的方法建造哮喘模型,模型组只建模,不予针刺干预；针刺组在建模的同时取“大椎”“肺俞”和“风门”进行针刺干预,隔日1次,共治疗7次(2周)；空白组不予任何处理.各组大鼠2周后使用肺灌洗法收集BALF,在光学显微镜下计数白细胞总数,瑞氏染色法计数EOS总数.结果:造模后,模型组大鼠BALF中白细胞、EOS计数与空白组比较明显升高(P＜0.05)；针刺后,针刺组大鼠BALF中白细胞、EOS计数与模型组相比明显降低(P＜0.05).模型组大鼠的BALF中EOS占白细胞比例高于空白组(P＜0.05)；针刺组大鼠BALF中的EOS占白细胞比例低于模型组(P＜0.05).结论:针刺干预能明显减少哮喘模型大鼠支气管肺泡灌洗液中白细胞和嗜酸性粒细胞数目,从而达到缓解哮喘的目的.

Impacts of acupuncture on inflammatory cells in BALF for the asthma model rats

Objective To explore the impacts of acupuncture on leukocyte （LEU） and eosinophilic granulocyte （EOS） in bronchoalveolar lavage fluid （BALF） in asthma model rats. Methods Thirty SPF-grade male SD rats were randomized into a blank group, a model group and an acupuncture group, 10 rats in each one. In the acupuncture group and the model group, the intraperitoneal injection of ovalbumin （OVA） was adopted to establish asthma model. In the model group, only the animal model was established but no intervention of acupuncture was given, in the acupuncture group, at the same time of the model establishment, acupuncture was provided at Dazhui （大椎 GV 14）, Feishu （肺俞 BL 13） and Fengmen （风门 BL 22）. The intervention was given once every two days, totally 7 treatments were required （2 weeks）. In the blank group, no any management was applied. The lung lavage method was adopted to collect BALF after 2 weeks for the rats in each group. Under optical microscope, the total LEU count was calculated. With Wright＇s staining, the total EOS count was calculated. Results In the model group, LEU and EOS counts in BALF in the rats were increased apparently compared with the blank group （both P〈0.05）. LEU and EOS counts in BALF in the rats in the acupuncture group were reduced apparently compared with the model group （both P〈0.05）. In BALF, the proportion of EOS in LEU in the rats in the model group was higher than that in the blank group （P〈0.05）. In BALF, the proportion of EOS in LEU in the rats in the acupuncture group was lower than that in the model group （P〈0.05）. Conclusion Acupuncture intervention reduces apparently LEU and EOS counts in BALF in asthma model rats so that asthma is relieved.