| 三氧化二砷诱导胶质母细胞瘤细胞凋亡作用的研究 |
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| 引用本文： | 李东海,淦作松.三氧化二砷诱导胶质母细胞瘤细胞凋亡作用的研究[J].中国临床神经外科杂志,2006,11(11):674-677. |
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| 作者姓名： | 李东海 淦作松 |
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| 作者单位： | 1. 南昌大学第一附属医院神经外科,江西,南昌,330006
2. 九江市第一人民医院神经外科,江西,九江,332000 |
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| 摘 要： | 目的探讨三氧化二砷(As2O3)及其联合地塞米松(DEX)诱导胶质母细胞瘤细胞凋亡的作用。方法实验分为空白对照组、As2O3组、DEX组、As2O3 DEX组,经倒置显微镜观察、吖啶橙荧光染色、流式细胞计数、细胞DNA片段断裂率来检测As2O3及其联合DEX诱导胶质母细胞瘤细胞凋亡的作用。结果As2O3以浓度依赖形式诱导BT-325细胞凋亡;DEX具有明显的协同效应。结论As2O3与DEX联合用药可能成为治疗胶质母细胞瘤的一条新途径。
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| 关 键 词： | 胶质母细胞瘤 三氧化二砷 凋亡 地塞米松 |
| 文章编号： | 1009-153X(2006)11-0674-04 |
| 收稿时间： | 04 17 2006 12:00AM |
| 修稿时间： | 06 15 2006 12:00AM |
Effect of Arsenic Trioxide on Cells Apoptosis of Glioblastomas |
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| LI Dong-hai,GANG Zuo-song.Effect of Arsenic Trioxide on Cells Apoptosis of Glioblastomas[J].Chinese Journal of Clinical Neurosurgery,2006,11(11):674-677. |
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| Authors: | LI Dong-hai GANG Zuo-song |
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| Institution: | Department of Neurosurgery, The First Affiliated Hospital, Nanchang University, Nanchang Jiangxi 330006, China |
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| Abstract: | Objective To evaluate the curative effects of arsenic trioxide (As2O3) on cells apoptosis of human glioblastoma cell line, BT-325 in vitro. Methods According to different agents added in the medium of cultured BT-325 cells, cultured glioblastoma cells were divided into As2O3, dexamethasone (DEX), As2O3 DEX and blank control groups. Cells apoptosis was assessed by inversion microscope observation, fluorescent staining, flow cytometry and cell DNA fragmentation rate analysis. Results As2O3 induced effectively apoptosis of BT-325 cells in a dose-time dependent manner. Significant synergetic effect of As2O3 and DEX on BT-325 cells apoptosis was observed. Conclusion Administration of As2O3 in combination with DEX may be a new method to treat of glioblastoma. |
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| Keywords: | Glioblastoma Arsenic trioxide (As2O3) Apoptosis Dexamethasone |
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