氯化镉致小鼠胸腺病理及酶组织化学改变

Changes in histopathology and enzyme histochemistry of thymus in cadmium exposure mice

The changes in histopathology and enzyme histochemistry of thymus induced by a single intraperitoneal injection of sublethal doses of cadmium chloride into Kunming male mice were examined. The swollen endothelium of capillaries was observed, with an obviously decreased activity of ICDH, LDH and ATPase, which seemed to be due to direct inhibition by cadmium at the 4th hour. The necroses of the cortex thymocytes were found at the 8th hour after injection and reached an extreme at the 16-24th hour, while few necroses of the lymphocytes in the medulla. Beginning 4th to 8th hour after exposure, the activity of enzymes was located in mitochondria of the cortex thymocytes, i.e., SDH, ICDH, CCO and ATPase, was decreased gradually. It suggested that thymic cortex had a marked impairment of blood supply and anoxia. Within 2 days after a single injection the cortex of the gland was mainly populated by epithelial reticular cells except a few lymphocytes. It was noted that there were some bigger cells which were characterized by their large size, basophilic cytoplasma, rough chromatin and high mitotic ability and activity of MDH, LDH, G-6-PD increased in these cells. From above observation the author concluded that the cause of cadmium-induced acute thymic atrophy was lymphocyte necroses within thymic cortex. The mechanism of the cortex thymocytes necrosis was possibly secondary to an anoxia of cortex resulting from capillary damage in the cortex. The ability of thymic regeneration is strong after being damaged. The regenerate cells possessed characteristics of morphology and enzyme histochemistry of immature cells, which probably came from the bone marrow.