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| 清脑滴丸对急性脑缺血海马MARCKS mRNA表达变化的调节作用 | |
| 引用本文： | 张允岭,张綦慧,白文,韩振蕴,郑宏,张锦,黄启福.清脑滴丸对急性脑缺血海马MARCKS mRNA表达变化的调节作用[J].中国中药杂志,2008,33(24):2938-2942. |
| 作者姓名： | 张允岭 张綦慧 白文 韩振蕴 郑宏 张锦 黄启福 |
| 作者单位： | 1. 北京中医药大学东方医院,北京,100078 2. 北京大学人民医院,北京,100044 3. 北京中医药大学基础医学院,北京,100029 |
| 基金项目： | 教育部高等学校博士学科点专项科研基金，教育部科学技术研究重点项目，教育部高等学校优秀青年教师教学科研奖励计划 |
| 摘 要： | 目的：观察清脑滴丸对急性脑缺血海马MARCKS mRNA表达变化的影响，探讨其对急性脑缺血损害（PKC-MARCKS）的调节作用。方法：采用同种系体外微栓子注入法复制急性多发脑梗死大鼠模型，将大鼠随机分为5组，正常组、假手术组、模型组、中药组、西药组，每组12只。正常组、假手术组和模型组给予等体积生理盐水灌胃，中药组和西药组分别给予清脑滴丸133．28mg·kg^-1，尼莫地平7．25mg·kg^-1。各组于造模前3d开始灌胃给药，每日1次。应用光学显微镜（HE染色）和透射电子显微镜观察急性脑缺血大鼠脑组织形态学改变，应用半定量PCR法检测各组患侧海马MARCKSmRNA表达水平。结果：正常组海马神经细胞紧密整齐，模型组、中药组和西药组神经细胞分别有不同程度受损，但中药组损伤程度相对较轻；模型组、中药组和西药组海马MARCKSmRNA均过高表达，但中药组明显低于模型组（P〈0．01）。结论：清脑滴丸可以减轻急性脑缺血时海马组织损伤，下调MARCKSmRNA的过高表达，其机制可能是通过调节PKC—MARCKS信号转导系统而起到神经保护作用。 |
| 关 键 词： | 清脑滴丸 急性多发脑梗死 MARCKS mRNA 调节作用 |
Regulation function of Qingnao drop pilula to MARCKS mRNA express changes in acute cerebral ischemia hippocampus |
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| ZHANG Yun-ling,ZHANG Qi-hui,BAI Wen,HAN Zhen-yun,ZHENG Hong,ZHANG Jin,HUANG Qi-fu.Regulation function of Qingnao drop pilula to MARCKS mRNA express changes in acute cerebral ischemia hippocampus[J].China Journal of Chinese Materia Medica,2008,33(24):2938-2942. | |
| Authors: | ZHANG Yun-ling ZHANG Qi-hui BAI Wen HAN Zhen-yun ZHENG Hong ZHANG Jin HUANG Qi-fu |
| Institution: | ZHANG Yun-ling1,ZHANG Qi-hui1,BAI Wen2,HAN Zhen-yun1,ZHENG Hong1,ZHANG Jin1,HUANG Qi-fu3(1.Dongfang Hospital,Beijing University of Chinese Medicine,Beijing 100078,China,2.People Hospital,Beijing University,Beijing 100044,3.School of Basic Medical Sciences,Beijing 100029,China) |
| Abstract: | Objective:To observe the contribution of Qingnao drop pilula to the alteration of myristoylated alanine-rich C kinase substrate(MARCKS) mRNA expression in acute multi-infarction hippocampus.Method: Rat models of acute multi-infarction were established by injecting the embolus of blood powder through the right external carotid arteryinto the internal carotid artery,rats were randomly divided into five groups(n=12 in each): normal,sham operation,model,Chinese medicine treatment,and Western medicine treatment.... |
| Keywords: | MARCKS mRNA |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |