白介素1β在大鼠卵泡发育中表达及功血宁Ⅱ号冲剂的影响*

目的] 观察白介素1β(IL-1β)在大鼠卵泡发育过程中的表达以及功血宁Ⅱ号冲剂对其表达的影响,探讨该药促进卵泡发育诱发排卵的作用机制.方法] 将未成年雌性大鼠随机分为模型对照组、阳性对照组和功血宁Ⅱ号高、低剂量组,分别给予蒸馏水、安坤赞育丸和功血宁Ⅱ号冲剂,14 d后皮下注射妊马血清促性腺激素(PMSG)+人绒毛膜促性腺激素(hCG)制备诱发排卵大鼠的模型.分别在卵泡发育期和排卵前期处死动物摘取卵巢,常规制备卵巢切片,免疫组化法检测各组动物卵泡不同发育时期IL-1β表达.结果] IL-1β在模型对照组中的表达,排卵前期的阳性细胞面积、阳性面积率、积分光密度、平均光密度均显著高于卵泡发育期(P＜0.05);功血宁Ⅱ号组在卵泡发育期和排卵前期1L-1β表达的上述指标均明显高于模型对照组(P＜0.05或P＜0.01),排卵前期的上述指标均明显高于相应卵泡发育期(P＜0.05或P＜0.01),其中高剂量组的作用较低剂量组和阳性对照组显著. 结论] IL-1β在大鼠卵泡发育过程中均有表达并逐渐增强.功血宁Ⅱ号冲剂能够显著提高IL-1β的表达,通过其参与卵母细胞成熟,诱导卵巢排卵因子的产生,在卵泡破裂激发排卵过程中发挥作用,促进卵泡发育诱发排卵.

Influence of supplementing Qi,activating blood flow and detoxicating on P-glucoprotein in colon tissue in patients with recurrent ulcerative colitis

Objective] To investigate the influence of supplementing Qi, activating blood flow and detoxicating treating on P-glucoproteinexpression in colon tissue of patients with recurrent ulcerative colitis (UC).Methods] Ninety cases with recurrent UC were randomlydivided into treatment group (n=45) and control group (n=45) according to the random table.After treatment for three months, the cases ofcomplete remission and of with effective treatment were followed-up for 6 months. (treatment group, n=39;control group, n=32).Thepatients of treatment group accepted decoction of supplementing Qi, activating blood flow and detoxicating treatment while the controlgroup used Salazosulfapyridine.Results] There were obvious differences between treatment group and control group.Before treatment,the level of colon tissue P-glucoprotein in UC group was much higher than that in health persons (P<0.01);the level of P-glucoprotein inrecurrent group was higher than that in non-recurrent group (P<0.01);but after treatment and follow-up period, the level of colon tissueP-glucoprotein in treatment group was lower than that in control group (P<0.01).Conclusion] The study shows that decoction ofsupplementing Qi, activating blood flow and detoxicating treatment for patients with recurrent UC can more effectively decrease the levelof colon tissue P-glucoprotein than treatment with Salazosulfapyridine.At the same time, the drug-resistance is restrained, and this canraise the curative effect and decrease the recurrence of the disease.