氯化锂联合自体激活雪旺细胞治疗脊髓损伤

目的 观察氯化锂联合移植自体激活雪旺细胞(AASCs)在治疗脊髓损伤(SCI)中的作用.方法 Wistar成鼠48只建立T10急性SCI模型.随机均分为4组:AASCs移植组、氯化锂移植组、氯化锂联合AASCs组和DMEM移植组.术后行体感诱发电位(SEP),运动诱发电位(MEP)检查和BBB评分.12周后进行10%BDA神经顺行示踪标记,切片后,行Cy3荧光探针染色、免疫组织化学及苏木素-伊红(HE)染色.结果 术后4周BBB评分各组之间差异开始有统计学意义(F=46.897,P<0.05).实验结束时,SEP、MEP波幅值各组间差异有统计学意义(F-SEP=15.47,P<0.05;F-MEP=8.974,P<0.05);BDA示踪,HE和免疫组织化学染色各组间差异有统计学意义(P<0.05).结论 氯化锂联合AASCs移植可以显著改善SCI后的功能恢复.

The study on repair of spinal cord injury by cografting auto Iogous activated schwann cells and lithium chloride

Objective To discuss the curative effectivenss of lithium chloride in combined with autologus activated Schwann cells (AASCs) in the treatment of spinal cord injury (SCI) in rats. Methods SCI models in 48 adult Wistar rats were made at T10 and divided into 4 groups:AASCs group,lithium chloride group, AASCs + lithium chloride group and DMEM group. After surgical operation, SEP, MEP and BBB score were made in every week. 10% BDA anterograde tracing mark, Cy3 fluorescence staining, HE staining and immunohistochemistry staining were made 12 months later. Results On the 4th week after grafting, there was statistically significant difference in BBB score between groups (ANOVA, F = 46.897, P < 0.05). At the end of the experiment, there was statistically significant difference in in SEP and MEP amplitudes of wave between groups (F-SEP = 15.47, P < 0.05 ; F-MEP = 8. 974, P < 0.05). Statistically significant difference was found in BDA tracing, HE and immunohistochemistry staining (P < 0.05). Con-clusion Evident amelioration after SCI can be found by co-transplanting AASCs and lithium chloride.