Molecular characterisation of extended-spectrum β-lactamase-producing Escherichia coli and Klebsiella spp. isolates at a tertiary-care centre in Lebanon |
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| Authors: | S. S. Kanj J. E. Corkill Z. A. Kanafani G. F. Araj C. A. Hart R. Jaafar G. M. Matar |
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| Affiliation: | Division of Infectious Diseases, American University of Beirut Medical Center, Beirut, Lebanon;, Department of Medical Microbiology, University of Liverpool, Liverpool, UK;, Department of Pathology &Laboratory Medicine;and Department of Microbiology and Immunology, American University of Beirut Medical Center, Beirut, Lebanon |
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| Abstract: | The prevalence of bla CTX-M, bla TEM and bla SHV genes among extended-spectrum β-lactamase (ESBL)-producing clinical isolates of Escherichia coli ( n = 50) and Klebsiella spp. ( n = 50) from Lebanon was 96%, 57% and 67%, and 40%, 82% and 84%, respectively. Genotyping revealed that the clonal diversity was unrelated to the presence of bla genes. Sequence analysis of 16 selected isolates identified the bla CTX-M-15, bla TEM-1, bla OXA-1 and six bla SHV genes, as well as the gene encoding the quinolone-modifying enzyme AAC(6')-Ib-cr. The genes encoding CTX-M-15 and AAC(6')-Ib-cr were carried on a 90-kb plasmid of the pC15-1a or pCTX-15 type, which transferred both ESBL production and quinolone resistance from donors to transconjugants. |
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| Keywords: | AAC(6')-Ib-cr Escherichia coli extended spectrum β-lactamase Klebsiella spp. plasmid quinolone resistance |
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