| 高效液相色谱法测定人血浆中西洛他唑浓度 |
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| 引用本文： | 李萍,温预关,何倩. 高效液相色谱法测定人血浆中西洛他唑浓度[J]. 中国药房, 2005, 16(18): 1404-1405 |
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| 作者姓名： | 李萍 温预关 何倩 |
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| 作者单位： | 1. 广州市脑科医院,广州市,510370
2. 武警广东总队医院,广州市,510507 |
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| 摘 要： | 目的:建立以高效液相色谱法测定人血浆中西洛他唑浓度的方法。方法:色谱柱为C18,流动相为甲醇-0.03mol/L醋酸铵(62∶38,V/V),流速为0.8ml/min,检测波长为257nm,以乙酸乙酯为提取剂。结果:西洛他唑检测浓度在10.0~1500.0ng/ml范围内线性关系良好(r=0.9999),高、中、低系列浓度的平均回收率分别为99.82%、97.30%、97.14%,日内和日间RSD均小于6%。结论:本方法灵敏、准确、简单、快速,可用于临床血药浓度监测和药动学研究。
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| 关 键 词： | 西洛他唑 血药浓度 高效液相色谱法 |
| 文章编号： | 1001-0408（2005）18-1404-02 |
| 收稿时间： | 2004-11-22 |
| 修稿时间： | 2005-01-24 |
Determination of Cilostazol in Human Plasma by HPLC |
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| LI Ping,WEN Yuguan,HE Qian. Determination of Cilostazol in Human Plasma by HPLC[J]. China Pharmacy, 2005, 16(18): 1404-1405 |
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| Authors: | LI Ping WEN Yuguan HE Qian |
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| Affiliation: | 1.Guangzhou Brain Hospital, Guangzhou 510370, China; 2.General Hospital of Armed Police Force of Guangdong Province, Guangzhou 510507, China |
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| Abstract: | OBJECTIVE:To establish a HPLC method for the determination of cilostazol in human plasma.METHODS:C18 was the chromatographic column,MeOH-0.03mol/L ammonium acetate (62∶38,V/V)was the mobile phase with a flow rate at 0.8 ml/min and detection wavelength at 257 nm,acetic ether was used as the extraction solvent.RESULTS:Good linear relationship was achieved when the detection concentration range of cilostazol was 10.0～1 500.0 ng/ml(r=0.9 999),the average recovery rates for the high,medium and low concentrations of cilostazol were 99.82%,97.30%and 97.14%,respectively,both the intra-day and inter-day RSD were lower than 6%.CONCLUSION:This method is sensitive,accurate,simple and fast,which can be used as the clinical monitoring of blood concentration of cilostazol and its pharmacokinetics study. |
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| Keywords: | Cilostazol Blood concentration HPLC |
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