miRNA145联合二甲双胍对人结直肠癌细胞株HCT116增殖的影响

目的:探讨miRNA145联合二甲双胍对人结直肠癌细胞株HCT116增殖的影响?方法:慢病毒法将miRNA145转染入人结直肠癌细胞株HCT116,实时定量PCR检测细胞miRNA145表达的变化?通过CCK-8细胞增殖实验检测miRNA145和/或二甲双胍对HCT116细胞增殖的影响?通过Western blot检测miRNA145和/或二甲双胍对细胞AMPK?mTOR蛋白表达水平的影响?结果:CCK-8实验表明miRNA145?二甲双胍均具有抑制HCT116细胞增殖的作用,二者联用使细胞增殖受到更大程度的抑制?Western blot结果表明,二甲双胍?miRNA145以及二者联用可进一步促进AMPK的活化进而影响mTOR蛋白表达水平?结论:miRNA145联合应用二甲双胍具有更强的抑制人结肠癌细胞株HCT116增殖的作用?

The effects of miRNA145 combined with metformin on the proliferation of human HCT116 cancer cells

Objective:To investigate the effects of miRNA145 combined with metformin on the proliferation of human HCT116 cancer cells. Methods:miRNA145 overexpression mediated by recombinant lentivirus was exployed in HCT116 cells. Cell proliferation assay by CCK-8 method was conducted to determine the roles of miRNA145 and/or metformin on the cancer cells. Western blot analysis was used to determine the expression levels of AMPK and mTOR proteins. Results:Both miRNA 145 and metformin can inhibit the cell proliferation of HCT116. The combination of miRNA 145 and metformin exerted a much stronger inhibitory effect on cell proliferation than any of the two. The activation level of AMPK was also higher by the combination,and the p-mTOR expression level was lower by the combination. Conclusion:miRNA145 and metformin can exert an anticancer effect synergistically.