转染TSLC1基因肝癌细胞株BEL-7402生物学特性的研究

目的 将人TSLCl基因转染人肝癌细胞BEL-7402中稳定表达,检测转染后细胞生物学特性的变化.方法 用脂质体法将TSLCl基因的真核质粒表达载体pcDNA3.1(+)/TSLCl导人人肝癌细胞株BEL-7402中,G418筛选克隆,再以逆转录-聚合酶链反应(RTPCR)、Western blot的方法检测TSLCI的表达,细胞计数、流式细胞术及裸鼠负荷瘤实验分析转染后细胞的生物学特性变化.结果 TSLCl在BEL-7402细胞中的表达可抑制BEL-7402细胞的生长,BEL-7402-TSLCI与BEL-7402比较s期及G2/M期的细胞减少,G0/G1期的细胞增加.转染后的BEL-7402-TSLCl细胞的凋亡明显增加,转染后荷瘤裸小鼠肿瘤的生长受到抑制.结论 TSLCl基因的转染可以抑制BEL-7402细胞的增殖、诱导肝癌细胞的凋亡,为肝癌的基因治疗提供理论依据.

Trausfection of TSLC1 in Human BEL-7402 cell line and its biological characteristics

Objective To transfect TSLC1 gene into human BEL-7402 hepatocellular carcinoma cell line and analyze biological characteristics of BEL-7402 cells after transfection.Methods TSLC1 full length cDNA was transfected into BEL-7402 cell line by the vector of pcDNA3.1 (+)/TSLC1 and the transfectant with stable expression of TSLC1 was obtained by clone selection.Effects of TSLC1 transfeetion on the biological characteristics of BEL-7402 cells were analyzed,including the changes in cell cycle,apoptosis and rate of growth.The BEL-7402 cells were injected into nude mice,and tumor formation at the sites of injection was monitored.Results The expression of TSLC1 in BEL-7402 cells inhibited the growth of hepatoma cells.The cell cycle analysis showed that TSLC1 transfected BEL-7402 cells could decrease the number of cells in S and G2/M phases,and increase the number of ceils in G0/G1 phase and the number of apoptosis cells arrested in the S phase.The apoptosis analysis indicated that TSLC1 transfection changed the apoptosis rate in BEL-7402 cell lines.TSLC1 inhibited the growth of human hepatocellular carcinoma ceils BEL-7402 in nude mice.Conclusion Transfection of TSLC1 inhibited growth and proliferation and induce apoptosis of hepatoma ceils BEL-7402.It may be used in gene therapy for hepatocellular carcinoma.