雷公藤药材总生物碱含量及影响因素的研究

目的:考察雷公藤药材总生物碱的含量状况及其变化规律。方法:采用有机溶剂提取和氧化铝柱色谱分离进行样品前处理,紫外分光光度法在268 nm波长下测定吸收度。结果与结论:一般韧皮部含量是木质部含量的2~5倍,二者含量变化呈现一致性。从不同种和类型看,昆明山海棠含量较高,雷公藤F次之,东北雷公藤最低;但每一类型内,不同居群间差别较小。含量较高的居群均来自雷公藤F和昆明山海棠,它们是江西遂川、安徽黄山、浙江景宁和云南昆明。居群内个体间的差别在不同居群中程度有所不同,浙江景宁、福建泰宁、云南昆明等居群内多样性丰富,有利于优良种质的选择。在同一植株内,药材粗细对总生物碱的含量影响不大。木质部总生物碱的含量呈现随季节变化的规律,3月含量最高,然后降低,12月降为最低。韧皮部的总生物碱含量变化规律不明显。

Study on total alkaloids content and its influential factors in medicinal materials of Tripterygium

Objective: To investigate the total alkaloids content profile and diversity of medicinal materials of Tripterygium/.Method: The total alkaloids were extracted by organic solvent and purified by column chromatography(aluminium oxide),and determined by UV spectrometry.Result and conclusion:!The total alkaloids content in phloem was 2 to 5 times richer than that in xylem,and their changes had shown concordance.For the different species/types,the content from high to low in order was T.hypoglaucum,Tripterygium F,T.wilfordii and T.regellii,but in each type,the disparity among different populations' content was very small.The populations with higher content were in Suichuan,Jiangxi Province,Huangshan,Anhui Province,Jingning,Zhejiang Province and Kunming,Yuannan Province;which came from T.hypoglaucum and Tripterygium F.The degree of difference of total alkaloids content among individuals in each population was different.The populations,which had a rich content diversity,were in Jingning,Zhejiang Province,Taining,Fujian Province and Kunming,Yunnan Province.They will be used for good germplasm selecting.The thickness of medicinal materials did not affect the total alkaloids content in the same plant.The total alkaloids content in xylem changed with seasons: the highest in March,then declining from June to December,the lowest in December,While this did not happen in phloem.