针刺血清不同组份对高嗜酸粒细胞血症大鼠外周血嗜酸粒细胞数目的影响

目的:在对针刺全血清进行预初分段分离的基础上,进一步分析、鉴定针刺血清中抗哮喘有效成分的大体所在.方法:将稀释后的分段血清以2.5mL/kg剂量经大鼠尾静脉注射到高嗜酸粒细胞血症模型大鼠的体内,连续注射3 d,同时连续10 d测定分段血清对高嗜酸粒细胞血症大鼠外周血嗜酸粒细胞计数的影响.结果:针刺血清组份1、组份2和组份3在尾静脉注射后3～10 d均可明显降低高嗜酸粒细胞血症大鼠的外周血嗜酸粒细胞计数(P＜0.05).结论:针刺血清中抗哮喘有效成分不是单一的,针刺可能激发了体内多种生物活性因子.

Effects of different segments of acupuncture serum on eosinophil counts in the rat with eosinophilia

Objective To further analyze and identify effective components of anti-asthma in acupuncture serum. Methods Changes of eosinophils in the peripheral blood of rats with eosinophilia were observed for 10 days after intravenous injection of the different segments of serum (serum:normal saline=1:20, 2. 5 mL/kg, from the first day of the model establishment, for 3 consecutive days). Results After intravenous injection of different segments of serum, the eosinophil counts in the peripheral blood decreased significantly from the 3rd day as compared with those of the model group (P<0. 05). Conclusion The effective components of acupuncture serum from asthmatic rats treated by acupuncture for eosinophils are not a single component, and acupuncture stimulation may produce many kinds of components of anti-asthma.