A competitive inhibition test of enzyme immunoassay for the anti-nRNP antibody |
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| Authors: | T. Nishimaki K. Sagawa S. Motogi K. Saito T. Morito H. Yoshida R. Kasukawa |
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| Affiliation: | Fukushima Medical College, Fukushima, Japan |
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| Abstract: | A competitive inhibition test for anti-nRNP antibody was developed, using horseradish-peroxidase-labelled anti-nRNP IgG derived from the serum of a patient with mixed connective tissue disease. In this test, the anti-nRNP antibody was clearly distinguished from the anti-Sm antibody in the patients' sera, and the sensitivity of the assay for anti-nRNP antibody was close to 50 ng/ml. |
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| Keywords: | Anti-nRNP antibody Enzyme immunoassay Rheumatic disease |
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