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| Allelotyping for loss of heterozygosity on chromosome 18 in gastric cancer | |
| 引用本文： | Yu JC,Sun KL,Liu B,Fu SB. Allelotyping for loss of heterozygosity on chromosome 18 in gastric cancer[J]. World journal of gastroenterology : WJG, 2004, 10(13): 1964-1966. DOI: 10.3748/wjg.v10.i13.1964 |
| 作者姓名： | Yu JC Sun KL Liu B Fu SB |
| 基金项目： | 国家自然科学基金,黑龙江省科研项目 |
| 摘 要： | |
| 关 键 词： | 等位基因型 杂合性 丢失基因 染色体18 胃癌 肿瘤 PCR 多态性 |
| 收稿时间： | 2003-12-23 |
Allelotyping for loss of heterozygosity on chromosome 18 in gastric cancer |
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| Yu Jing-Cui,Sun Kai-Lai,Liu Buo,Fu Song-Bin. Allelotyping for loss of heterozygosity on chromosome 18 in gastric cancer[J]. World journal of gastroenterology : WJG, 2004, 10(13): 1964-1966. DOI: 10.3748/wjg.v10.i13.1964 | |
| Authors: | Yu Jing-Cui Sun Kai-Lai Liu Buo Fu Song-Bin |
| Affiliation: | 1. Department of Clinical Pharmacology,the Second Affiliated Hosptial,Harbin Medical University,Harbin 150086,Heilongjiang Province,China 2. Department of Medical Genetics,China Medical University,Shenyang 110001,Liaoning Province,China 3. Chinese National Human Genome Center,Beijing 100176,China 4. Laboratory of Medical Genetics,Harbin Medical University; Bio-pharmaceutical Key Laboratory of Heilongjiang Province,Harbin 150086,Heilongjiang Province,China |
| Abstract: | AIM: To investigate the association between loss of heterozygosity (LOH) on chromosome 18 and sporadic gastric cancer. METHODS: Multiplex PCR was used to screen 14 highly polymorphic microsatellite markers on chromosome 18 in 45 cases of primary gastric cancer. PCR products were separated on polyacrylamide gels and the electrophoresis maps were analyzed with Genescan and Genotyper. RESULTS: The LOH frequencies in gastric cancer at all 14 markers ranged from 10% to 58%. Eleven markers were found with over 20% LOH frequencies, in which 9 markers located in 18q, and 2 markers in 18p. Two overlapping deleted regions were identified: R1 between D18S61-D18S1161 at 18q22 (9cM) with 24% LOH frequency; R2 between D18S462-D18S70 at 18q22-23(6cM) with 32% LOH frequency. CONCLUSION: LOH of chromosome 18 (18q and 18p) may be involved in gastric tumorigenesis. Two overlapping deleted fragments suggested that there might be unidentified tumor suppressor genes in those two regions. |
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