| Homer1在糖尿病大鼠胰腺中的表达及意义 |
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| 引用本文： | 许莹,许淑芳,吴三云,涂建成. Homer1在糖尿病大鼠胰腺中的表达及意义[J]. 武汉大学学报(医学版), 2012, 33(3): 332-335 |
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| 作者姓名： | 许莹 许淑芳 吴三云 涂建成 |
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| 作者单位： | 许莹 (武汉市第五医院内分泌科,湖北武汉,430050) ； 许淑芳 (湖北省黄冈市中心医院内分泌科,湖北黄冈,438000) ； 吴三云 (武汉大学中南医院检验科,湖北武汉,430071) ； 涂建成 (武汉大学中南医院检验科,湖北武汉,430071) ； |
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| 摘 要： | 目的:观察Homer1在大鼠胰腺中的表达,探讨Homer蛋白的生理意义。方法:建立2型糖尿病大鼠模型,与正常大鼠比较,检测血胰岛素值。取胰腺组织,检测Homer1a、Homer1c和胰岛素表达情况。结果:Homer1a、Homer1c表达于大鼠胰腺组织中胰岛细胞,糖尿病大鼠胰腺组织与正常组大鼠比较,胰岛素表达明显减少(P<0.01),Homer1a表达减少(P<0.05),Homer1c表达增加(P<0.05)。结论:Homer蛋白表达于大鼠胰腺组织;且在糖尿病模型中,Homer1c的表达与Homer1a的表达方向相反,Homer1蛋白可能参与胰岛素分泌的调控机制。
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| 关 键 词： | Homer1 胰腺 表达 |
Expression of Homer1 Protein in Pancreas of Diabetic Rats |
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| XU Ying,XU Shufang,WU Sanyun,TU Jiancheng. Expression of Homer1 Protein in Pancreas of Diabetic Rats[J]. Medical Journal of Wuhan University, 2012, 33(3): 332-335 |
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| Authors: | XU Ying XU Shufang WU Sanyun TU Jiancheng |
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| Affiliation: | 1Dept.of Endocrinology,The Fifth Hospital of Wuhan,Wuhan 430050,China 2Dept.of Endocrinology,Huanggang Central Hospital,Huanggang 438000,Hubei,China 3Laboratory Center,Zhongnan Hospital of Wuhan University,Wuhan 430071,China |
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| Abstract: | Objective: To study the expression of Homer 1 protein in pancreas of rats with diabetic mellitus.Methods: Homer1(Homer1a/c) expression was detected both in mRNA and protein levels in the pancreas tissue of 20 diabetic rats and 20 normal controls by using PCR and immunohistochemistry methods.Serum insulin levels were also detected in both groups.Results: Homer1a/c protein was positively expressed in the pancreas of both groups.In rats with diabetic mellitus,serum insulin levels and the expression of Homer1a was much lower than that respectively in the control group,while the expression of Homer1c were much higher.Conclusion: The result shows that there is a significant change in the expression of Homer1a/c and insulin between the two groups.Homer1 protein may play important roles in the modulation of pancreas endocrine. |
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| Keywords: | Homer1 Pancreas Expression |
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