重组人生长激素对大鼠随意皮瓣成活的影响

目的探讨重组人生长激素(rhGH)对大鼠随意皮瓣成活的影响。方法将24只健康的Wistar大鼠随机分为两组,每组12只。在其背部制作随意皮瓣后,分别在皮瓣的皮下注射0.25ml(0.1IU/kg)的rhGH(实验组)和0.25ml的生理盐水(对照组),观察两组皮瓣的成活面积及成活率,并行免疫组化检查。结果实验组的皮瓣成活率及皮瓣的中段、远段的血管密度和VEGF的表达均较对照组高,实验组皮瓣血管的再生明显多于对照组。结论rhGH能直接或间接地促进皮瓣血管的生成,提高随意皮瓣成活的面积。

Effect of recombinant human growth hormone on survival of free skin flaps in rats

Objective To study the function of recombinant human growth hormone (rhGH) on the survival of the free skin flaps in rats. Methods Twenty-four Wistar rats were randomly divided into two groups with 12 rats in each group, and a free dorsal skin flap was established in each animal. In the experimental group each rat was given a subcutaneous injection with rhGH (0.1 IU/kg, total volume 0.25 ml) under the skin flap and the control group animals each received a saline injection under the flap of the same volume. The viable area and survival rate of all flaps were observed, and an immunohistochemical test was performed. Results The flap survival rate in the experimental group was remarkably higher than that in the control group. The blood vessels density in the middle and distal segments of the flap was greater and the expression of VEGF in the experimental group was stronger than that in the control group. Histological semiquanti tative analysis showed neovascularizations in flaps treated with rhGH was much greater than that in the control group. Conclusion rhGH can directly or indirectly promote vascularization and increase both the flap survival rate and area viability of the free skin flaps.