Angiostatin的分离纯化及对B16黑色素瘤的抑制作用

目的:研究Angiostatin对黑色素瘤的治疗作用.方法:从人血浆组分Ⅲ中用亲和层析的方法分离Plasminogen和通过离子交换层析和分子筛技术得到弹性蛋白酶纯品.经此酶分离Plasminogen进行有限酶解,产物经亲和层析分离得到Angio-statin.结果:体外实验表明,Angiostatin能显著抑制牛主动脉弓内皮细胞的增殖.体内实验发现,以0.6 mg/kg·d的剂量腹腔注射14d后,B16黑色素瘤生长抑制率达77%(P<0.05);以小鼠尾静脉注射的方法建立黑色素瘤转移模型,采用同样的方法冶疗18d后,B16黑色素瘤的肺转移率降低了65%(P<0.05).结论:本研究为Angiostatin用于黑色素瘤的治疗提供了实验依据,说明Angiostin在肿瘤治疗方面有着广泛的应用前景.

Purification of Angiostatm and Its Inhibitory Effects on B16 Melanoma

Objective: To investigate the efficiency of treatment with angiostatin on melanoma. Methods: Angiostatin was purified through affinity chromatography from human plasminogen fragments obtained by digesting human plasminogen with elastase. Results: The result of experiment in vitro showed that the purified angiostatin could significantly inhibit the proliferation of bovine aortic endothelial cells stimulated by bFGF. Systematic administration of angiostatin at does of 0.6 mg/kg d for 14 days, the tumor weight reduced about 77 % ( P < 0.05) . We established the melanoma metastasis model by injection of B16 cells through mouse tail vein. Under the same treatment for 18 days, the melanoma lung metastasis reduced about 65 % ( P < 0.05) . Conclusion: Our results supply the experimental evidence for the application of angiostatin in melanoma therapy.