| 表达Angiostatin基因的B16黑色素瘤体内抑制作用 |
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| 引用本文： | 夏国宏,卢卫新,邢力,费俭,郭礼和. 表达Angiostatin基因的B16黑色素瘤体内抑制作用[J]. 中国肿瘤生物治疗杂志, 1999, 6(4): 284-287 |
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| 作者姓名： | 夏国宏 卢卫新 邢力 费俭 郭礼和 |
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| 作者单位： | 中国科学院上海细胞生物学研究所!上海200031 |
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| 摘 要： | 目的:研究表达Angiostatin基因的B16黑色素瘤的体内生长行为.方法:通过改造Plasminogen cDNA得到Angiostatin基因,构建成真核表达载体pAGAGS3后导入B16黑色素瘤细胞使其表达.结果:表达Angiostatin的B16黑色素瘤细胞体外生长速率和软琼脂中克隆形成能力均未改变.但接种小鼠26d后,体内生长抑制率达87%(P<0.01).结论:表达Angiostatin的B16黑色素瘤体内生长受到显著抑制.
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| 关 键 词： | B16黑色素瘤 Angiostatin 基因表达 血管生成 |
| 收稿时间： | 1998-09-07 |
| 修稿时间： | 1998-11-12 |
Inhibitory Efficiency on Growth in vivo of B16 Melanoma Cell Expressing Angiostatin |
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| Xia Guohong,Lu Weixin,Xing Li,Fei Jian and Guo Lihe. Inhibitory Efficiency on Growth in vivo of B16 Melanoma Cell Expressing Angiostatin[J]. Chinses Journal of Cancer Biotherapy, 1999, 6(4): 284-287 |
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| Authors: | Xia Guohong Lu Weixin Xing Li Fei Jian Guo Lihe |
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| Affiliation: | Shanghai Institute of Cell Biology, Chinese A-cademy of Sciences , Shanghai 200031;Shanghai Institute of Cell Biology, Chinese A-cademy of Sciences , Shanghai 200031;Shanghai Institute of Cell Biology, Chinese A-cademy of Sciences , Shanghai 200031;Shanghai Institute of Cell Biology, Chinese A-cademy of Sciences , Shanghai 200031;Shanghai Institute of Cell Biology, Chinese A-cademy of Sciences , Shanghai 200031 |
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| Abstract: | Objective: Study on the growth character of B16 melanoma cell which can express angiostatin. Methods: Angiostatin gene was constructed from human plasminogen cDNA by deletion mutation. A B16 melanoma cell clone named BAG28 which stably expresses angiostatin was established by introducing gene into it. Results: BAG28 in vitro had no changes in proliferation rate and the ability of clone formation in soft agar. Study in vitro showed that the tumor weight had reduced about 87% ( P < 0. 01 ) 26 days after inoculation in mouse. Conclusion: The growth of B16 melanoma cells expressing angiostain was significandy inhibited in vivo. |
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| Keywords: | B16 melanoma angiostatin gene expression angiogenesis |
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