大鼠坐骨神经挤压伤后基质金属蛋白酶-2和-9的表达及意义

目的观察大鼠坐骨神经挤压伤后基质金属蛋白酵（MhiP）-2和-9的表达，及其随损伤后恢复时程的变化。方法建立大鼠坐骨神经挤压伤模型，用明胶酶谱测定损伤后神经组织内btMP-2和bthiP-9的活性变化；Westernblot检测确认所消化条带的属性。结果正常坐骨神经组织内仅表达MMP-2，不表达MMP-9；坐神经挤压伤后1d时，MMP-9表达水平最高；MMP-2在坐骨神经损伤后表达水平略有增加，7d时最高。Westernblot检测确认所消化的条带是MMP-2和MMP-9。结论MMP-2和MMP-9参与坐骨神经损伤后的损伤修复过程。

Expression of matrixmetalloproteinase in rats' sciatic nerves after extrusion wound

Objective To investigate expression of matrixmetalloproteinase(MMP)-2 and MMP-9 in rats' sciatic nerves after extrusion wound.Methods Constructed rat models with extrusion wound at the sciatic nerves,detected activity changes of MMP-2 and MMP-9 in the sciatic nerves by gelatinzymography,identified the attribution with Western blot.Results MMP-2 existed innormal sciatic nerves and sciatic nerves with extrusion wound,while MMP-9 was only detected in the latter.The expression of MMP-9 was the highest 1day after the extrusion wound,while that of MMP-2 was the highest 7days after the wound.Conclusion MMP-2 and MMP-9 may participate in the recovery process of sciatic nerves after extrusion wound.