Genome sequence comparison of two United States live attenuated vaccines of infectious laryngotracheitis virus (ILTV) |
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Authors: | Chandra Yohanna Gita Lee Jeongyoon Kong Byung-Whi |
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Institution: | (1) Department of Poultry Science, University of Arkansas, Fayetteville, AR 72701, USA;(2) Cell and Molecular Biology Program, University of Arkansas, Fayetteville, AR 72701, USA;(3) Center of Excellence for Poultry Science, University of Arkansas, POSC O-404, 1260 W. Maple Street, Fayetteville, AR 72701, USA; |
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Abstract: | This study was conducted to identify unique nucleotide differences in two U.S. chicken embryo origin (CEO) vaccines LT Blen
(GenBank accession: JQ083493) designated as vaccine 1; Laryngo-Vac? (GenBank accession: JQ083494) designated as vaccine 2] of infectious laryngotracheitis virus (ILTV) genomes compared to an
Australian Serva vaccine reference ILTV genome sequence Gallid herpesvirus 1 (GaHV-1); GenBank accession number: HQ630064]. Genomes of the two vaccine ILTV strains were sequenced using Illumina Genome Analyzer
2X of 36 cycles of single-end reads. Results revealed that few nucleotide differences (23 in vaccine 1; 31 in vaccine 2) were
found and indicate that the US CEO strains are practically identical to the Australian Serva CEO strain, which is a European-origin
vaccine. The sequence differences demonstrated the spectrum of variability among vaccine strains. Only eight amino acid differences
were found in ILTV proteins including UL54, UL27, UL28, UL20, UL1, ICP4, and US8 in vaccine 1. Similarly, in vaccine 2, eight
amino acid differences were found in UL54, UL27, UL28, UL36, UL1, ICP4, US10, and US8. Further comparison of US CEO vaccines
to several ILTV genome sequences revealed that US CEO vaccines are genetically close to both the Serva vaccine and 63140/C/08/BR
(GenBank accession: HM188407) and are distinct from the two Australian-origin CEO vaccines, SA2 (GenBank accession: JN596962)
and A20 (GenBank accession: JN596963), which showed close similarity to each other. These data demonstrate the potential of
high-throughput sequencing technology to yield insight into the sequence variation of different ILTV strains. This information
can be used to discriminate between vaccine ILTV strains and further, to identify newly emerging mutant strains of field isolates. |
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