运动训练对大鼠局灶性脑缺血后微血管新生的影响

目的：探讨运动训练对局灶性脑缺血后微血管新生的影响。方法：55只SD大鼠随机分为假手术对照组、模型组、康复组；模型组和康复组动物以线栓法制成大鼠左侧大脑中动脉梗死模型，康复组术后1天开始进行运动训练．每周5天，共4周；其余两组常规饲养。以免疫组织化学法检测血管内皮生长因子（VEGF）、因子表达，测定微血管密度。结果：大鼠大脑中动脉栓塞后，缺血区神经元变性、坏死，VEGF和因子在缺血周边区表达明显增加．经运动训练干预后，VEGF和因子表达大量增加，做血管数目明显增加。结论：运动训练可通过促VEGF表达上调．促进微血管新生。

Effects of rehabilitative training on VEGF expression and angiogenesis of rats with cerebral infarction

Objective:To evaluate the effects of rehabilitative training on VEGF expression and angiogenesis and its role in functional outcome after cerebral infarction in rats.Method:55 male SD rats were divided into control group, model group and rehabilitation group randomly. The rat models of focal cerebral infarction were established with unilateral middle cerebral artery(MCA) suture occlusion method in model group and rehabilitation group. The rats in rehabilitation group were given exercising everyday, while those in other groups were reared in their original living state.VEGF expression and angiogenesis was measured in the boundary zone to brain infarction at 1d, 3d, 7d, 14d, and 28d.Result:It was found that VEGF expression started at 1d, and subsequently increased, reached peak value at the 3d, failed away until 14d in model group and rehabilitation group.VEGF expression was mainly detected in the boundary zone of the infarcts at every timepoint. To compare with model group, VEGF expression of rehabilitation group was significantly increased in 3d and 7d after MCAO.Compare with control group, the microvessel density(MVD) in model group and rehabilitation group were significantly increased in 14d and 28d after MCAO, especially in rehabilitation group.Conclusion:Rehabilitative training can increase the expression and promote the blood vessel proliferation in rats with cerebral infarction.