赤芍总苷提取液体外清除自由基和抗凝血活性的研究

目的:研究赤芍总苷(TPG)提取液体外清除自由基和抗凝血的活性。方法:通过相同溶剂不同方法提取TPG,采用二苯基三硝基苯肼(DPPH)自由基体系、超氧阴离子自由基体系、羟基自由基体系检测TPG清除自由基的活性,同时测定TPG提取液对凝血酶原时间(PT)、部分凝血酶时间(APTT)、凝血酶时间(TT)的影响。结果:沸水浴0.5h提取2次,TPG含量为4.682%;采用不同方法得到的TPG提取液对DPPH清除率为92.36%～96.65%,抗超氧阴离子活力和活性氧活力分别为415.15～496.97U.L-1和774.32～1911.60U.L-1;当TPG浓度≥0.012g.L-1时可显著延长PT(P<0.05),当TPG浓度≥0.0012g.L-1时可显著延长APTT(P<0.05)和TT(P<0.01)。结论:采用不同方法得到的TPG提取液均有较强的清除自由基和抗凝血活性。

Study on the Free Radical-Scavenging and Anticoagulant Activities of Total Paeony Glycoside in Vitro

OBJECTIVE:To analyze and compare the free radical-scavenging and anticoagulant activities of Total paeony glycoside(TPG)extracts in vitro.METHODS:TPG was extracted with the same solvent but different extracting methods,and free radical-scavenging and anticoagulant effects of different extracts were detected by the assay systems of DPPH,superoxide radical and hydroxyl radical as well as PT,APTT and TT test.RESULTS:The extraction rate of TPG boiling in water twice was 4.682%.The effects on scavenging free radical were 92.36% ～ 96.65%(DPPH radical),415.15～ 496.97 U· L-1(superoxide radical)and 774.32～ 1 911.60 U· L-1(hydroxyl radical)respectively,and the TPG extracts also prolonged PT(TPG≥ 0.012g· L-1,P<0.05),APTT(TPG≥ 0.001 2g· L-1,P<0.05)and TT(TPG≥ 0.001 2g· L-1,P<0.01).CONCLUSION:TPG extracts have obvious free radical-scavenging and anticoagulant activities,but different extracting methods may influence the free radical-scavenging and anticoagulant activities and mechanism.