高功率微波对视网膜神经节细胞脂质过氧化作用的实验研究

目的 观察微波对体外培养的兔视网膜神经节细胞的脂质过氧化损伤作用。 方法 体外培养兔神经节细胞，以平均功率为80　mW/cm²的微波进行辐照，辐照时间分别为15、30、45min。辐照后立即于倒置显微镜和电镜下观察细胞形态变化，羟胺法和改良硫代巴比妥酸荧光法分别测定细胞内超氧化物歧化酶(superoxide dismutase,SOD)和丙二醛(malondialdehyde,MDA)含量。 结果 微波辐照后，细胞轴突消失，电镜可见线粒体及内质网肿胀。随辐照时间延长，细胞逐渐趋向于坏死，细胞MDA含量增高，45min辐照组MDA含量为对照组的5.11倍。SOD则逐渐降低。 结论 微波可引起视网膜神经节细胞脂质过氧化损伤，脂质过氧化反应可能是微波视网膜损伤的作用机制之一。(中华眼底病杂志，2000，16：32-34)

Study of lipid peroxide damage in retinal ganglion cells induced by high power microwave

Objective To determine the lipid peroxide damage in the primary cultured rabbit retinal ganglion cells induced by microwave. Methods Cultured rabbit retinal ganglion cells in vitro and exposed to 80 mW/cm 2 of microwave for 15,30,45 min tespectively.Immediately after radiation,the morphological variation of cells was observed by optical microscope and transmission electronic microscope.Secondly,the activity of intracellular superoxide dismutase (SOD) and the content of malondialdehyde (MDA) were detected. Results A portion of cells congregated,with their axon disapeared after radiation.Mitochondria and endoplasmic reticulum revealed swelling under transmission electronic microscope.The content of MDA was increased obviously compared with control group while SOD decreased.The content of MDA as increased obviously compared with control group after 45 min radiation was 5.11 times,while SOD decreased.The content of MDA as in control and the ganglion cells were apparantly destroyed. Conclusion Microwave can induce the lipid peroxide damage in primary cultured retinal ganglion cells,and lipid peroxide effect might be one of the mechanisms of microwave retinal damage.