热毒宁注射液解热作用与化学质控成分相关性研究

目的 探讨热毒宁注射液化学质控成分与解热作用的关系.方法 建立脂多糖(LPS)致大鼠发热动物模型,研究热毒宁注射液解热作用和对中枢发热介质前列腺素E2(PGE2)影响.复制LPS诱导巨噬细胞RAW264.7释放PGE2模型,并采用环氧化合酶(COX)-2活性实验,考察热毒宁注射液及其化学质控成分对PGE2释放和COX-...

Study on the Relationship Between the Antipyretic Activity and Chemical Quality Control Components of Reduning Injection

OBJECTIVE To explore the relationship between the antipyretic activity and chemical quality control components of Reduning Injection(RDN).METHODS RDN was administered on lipopolysaccharide(LPS)-induced fever rats to observe the antipyretic effect and detect the level of PGE 2 in hypothalamus tissue.LPS-induced RAW 264.7 cells were replicated and cyclooxygenase(COX)-2 enzyme assay were performed to evaluate the activity of RDN and its chemical components against PGE 2 and COX-2.RESULTS RDN could obviously reduce the temperature rise in fever rats,and PGE 2 level in hypothalamus tissues.RDN could also inhibit the release of PGE 2 and the activity of COX-2,with IC 50 of 27.07μg/mL,418.6 ng/mL,respectively.Meanwhile,the level of PGE 2 was reduced by caffeic acid,neochlorogenci acid and isochlorogenic acid A,isochlorogenic acid B,while the activity of COX-2 was inhibited by chlorogenic acid,crytochlorogenic acid,caffeic acid and isochlorogenic acid A,isochlorogenic acid B,isochlorogenic acid C.CONCLUSION RDN shows significant antipyretic effect via inhibiting the activity of COX-2 and reducing the release of PGE 2,which was related to caffeolyquinic acids in chemical quality control indexes.