红豆杉属植物中紫杉烷化合物含量比较与分析

目的 分析和比较5种红豆杉中6种紫杉烷类化合物含量的差异.方法 利用乙醇超声浸提后得到样品溶液;通过高效液相色谱法测定和相关性分析、回归分析和聚类分析比较紫杉醇、巴卡亭Ⅲ、10-去乙酰基巴卡亭Ⅲ(10-deacetylbaccatinⅢ,10-DAB)、10-去乙酰基紫杉醇(10-deacetyltaxol,10-DA...

Comparative, regression and cluster analysis on contents of six taxanes in Taxus spp.

Objective To analyze and compare the contents of six taxanes in five different Taxus species. Methods The sample solution was obtained by ultrasonic extraction with ethanol; The contents of taxol, baccatin III, 10-deacetylbaccatin III (10-DAB), 10-deacetyltaxol (10-DAT), cephalotaxine, and 10-deacetyl-7-xylosetaxol (DXT) were investigated by HPLC method, and analyzed with correlation, regression, and cluster analysis. Results The contents of 10-DAB, Taxol, 10-DAT and cephalotaxine in Taxus chinensis form Longnan Area were significantly higher than those in other Taxus species. The contents of 10-DAB T. chinensis var. mairei and T.×media were higher than the others. Moreover, with the correlation, regression, and cluster analysis results, 10-DAT and cephalotaxine were significantly correlated with taxol content, respectively. Furthermore, the cluster analysis also showed that the plants of T. chinensis var. mairei which planted in the greenhouse were clustered into one group, and the plants of T. chinensis, T. cuspidata, T. chinensis var. mairei from Taihang Mountain, and T.×media which planted in field were grouped together, indicating that environmental factors have obvious effects on taxanes accumulation. Conclusion Taxanes contents in different Taxus species are significantly different, and taxol content increases with the increase of 10-DAT and cephalotaxine contents.