基于网络药理学方法探讨八珍汤治疗ITP的作用机制＊

目的 采用网络药理学方法,探索八珍汤治疗原发免疫性血小板减少症（Primary immune thrombocytopenia, ITP）的作用机制。方法 通过中药系统药理学分析平台（TCMSP）、Drugbank数据库和文献检索方式获得八珍汤8味中药的化学成分和作用靶点,以口服吸收利用度和药物相似性等条件进行筛选。通过OMIM数据库、TTD数据库、GAD数据库和GeneCards数据库查找ITP的相关靶点。进而利用Cytoscape 3.7.1软件构建化学成分-靶点网络、疾病-靶点网络、成分靶点和疾病靶点交集网络,通过Cytoscape软件中ClueGO插件和DAVID在线分析工具对八珍汤治疗ITP的相关靶点进行基因本体（GO）功能富集分析和KEGG通路分析,探讨八珍汤治疗ITP的作用机制。结果 共筛选得到159个八珍汤化学成分和146个靶点;获得306个ITP相关靶点;得到21个八珍汤治疗ITP的作用靶点;显著富集到114个生物过程条目和34条KEGG生物通路。结论 八珍汤主要通过TNF信号通路、HIF-1信号通路、NOD样受体信号通路、VEGF信号通路和T细胞受体信号通路等发挥治疗ITP的作用,其中TNF信号通路可能是其关键机制。

Mechanisms of Bazhen Decoction in Treatment of Primary Immune Thrombocytopenia Based on Network Pharmacology

Objective To explore and investigate the mechanisms of Bazhen Decoction in the treatment of primary immune thrombocytopenia (ITP) by using network pharmacology approach.Methods Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform (TCMSP), Drugbank database, and literature retrieval were used to obtain chemical components and targets of Bazhen Decoction combined with oral bioavailability and drug analysis to screen the components. OMIM database, TTD database, GAD database, and GeneCards database were used to search for the ITP-related targets. Then the compound-target network, ITP-target network, and interaction network of Bazhen Decoction and ITP-related targets were constructed by using Cytoscape 3.7.1 software. Gene ontology (GO) and KEGG pathway analysis were carried out by using the ClueGO plug-in of Cytoscape software and Database for Annotation, Visualization and Integrated Discovery (DAVID) to analyze the mechanisms of Bazhen Decoction in the treatment of ITP.Results In total, 159 chemical components and 146 targets of Bazhen Decoction, and 306 ITP-related targets were obtained. In addition, 21 candidate targets of Bazhen Decoction against ITP, 114 terms of GO analysis, and 34 KEGG pathways were obtained.Conclusion Results suggested that TNF signaling pathway, HIF-1 signaling pathway, NOD-like receptor signaling pathway, VEGF signaling pathway, and T cell receptor signaling pathway were the key factors affecting the efficacy of Bazhen Decoction for treatment of ITP, in which TNF signaling pathway may be the key mechanism.