IL-33与绝经后骨质疏松女性骨密度和骨代谢指标相关性研究

目的 探索血清白细胞介素-33(IL-33)与绝经后骨质疏松女性骨密度和骨代谢指标相关性。方法 采用酶联免疫吸附法测定50例绝经后骨质疏松患者和50例正常绝经后妇女血清IL-33水平。采用双能X线骨密度仪(DXA)测量患者和对照组的骨密度(BMD)。检测维生素D、钙、碱性磷酸酶(ALP)、甲状旁腺激素(PTH)水平，以及1型胶原C末端肽(CTX)和1型前胶原N端前肽(P1NP)等骨转换指标。结果 在绝经后骨质疏松症女性中，IL-33水平显著低于健康对照组(3.53±2.45) pg/mL vs (13.72±5.39) pg/mL，P=0.007]；Spearman相关分析表明血清IL-33水平与年龄、BMI、PTH、CTX和P1NP水平呈负相关，与腰椎BMD和股骨颈BMD呈正相关。多元回归分析表明，年龄、BMI、腰椎BMD、PTH、股骨颈BMD和血清CTX和P1NP水平是骨质疏松症患者血清IL-33水平降低的独立预测因子。结论 血清IL-33降低是绝经后骨质疏松患者股骨颈和腰椎骨密度降低和骨转换增速的危险因素。

Correlation between serum IL-33 and bone mineral density and bone metabolism indexes in postmenopausal women with osteoporosis

Objective To explore the correlation between serum interleukin-33 (IL-33) and bone mineral density and bone metabolism indexes in postmenopausal women with osteoporosis. Methods In 50 postmenopausal osteoporotic patients and 50 healthy postmenopausal control women, serum IL-33 levels were measured using enzyme linked immunosorbent assay. In both patients and controls, bone mineral density (BMD) was measured usingduel-energy X-ray absorptiometry (DXA). Serum levels of vitamin D, calcium, alkaline phosphatase (ALP), parathyroid hormone (PTH) , as well as bone turnover markers, such as C-terminal telopeptide of type 1 collagen (CTX) and N-terminal propeptide of type 1 procollagen (P1NP) were also detected. Results In postmenopausal women with osteoporosis, the level of IL-33 was significantly lower than that in the healthy control group (3.53 ± 2.45 vs 13.72 ± 5.39 pg/mL; P=0.007). Spearman correlation analysis showed that serum IL-33 levels were negatively correlated to age, BMI, PTH, CTX, and P1NP, but were positively correlated with BMD of the lumbar vertebrae and femoral neck. Multiple regression analysis showed that age, BMI, lumbar BMD, PTH, femoral neck BMD, and serum CTX and P1NP levels were independent predictors of decreased serum IL-33 levels in patients with osteoporosis. Conclusion Decreased serum IL-33 is a risk factor for the reduction of BMD and the increase of bone turnover in femoral neck and lumbar vertebrae in postmenopausal osteoporosis patients.