红芪多糖对骨髓间充质干细胞和成骨细胞的影响

目的 研究3种新型红芪多糖(HPS-1、HPS-2、HPS-3)对大鼠骨髓间充质干细胞rBMSCs和颅骨成骨细胞ROBs成骨性分化的影响.方法 采用MTT法检测rBMSCs细胞和ROBs细胞增殖;采用碱性磷酸酶(alkaline phosphatase,ALP)试剂盒检测rBMSCs细胞和ROBs细胞的ALP活性;采用...

Effects of hedysarum polysaccharides on osteogenic differentiation of rBMSCs and ROBs

Objective To investigate the effects of hedysarum polysaccharides(HPS-1,HPS-2,HPS-3)on promoting osteogenic differentiation of rat bone marrow stromal cells(rBMSCs)and rat calvarial osteoblasts(ROBs).Methods MTT assay was used to detect the proliferation of rBMSCs and ROBs.Alkaline phosphatase(ALP)activity of rBMSCs and ROBs were detected by ALP kit.Mineralized nodule formation of rBMSCs and ROBs was detected by alizarin red S staining.Results HPS-1(20,50μg/mL)significantly promoted the proliferation of rBMSCs(P<0.05,0.01),HPS-1(20μg/mL)significantly promoted the proliferation of ROBs(P<0.01),HPS-2 and HPS-3 had no influence on promoting the proliferation of rBMSCs and ROBs.HPS-1(50,100μg/mL)significantly increased ALP activity in rBMSCs(P<0.01),HPS-1(10,20,50μg/mL)significantly increased ALP activity in ROBs(P<0.05,0.01),HPS-3(50μg/mL)significantly increased ALP activity in rBMSCs(P<0.05).HPS-2 had no influence on increasing ALP activity in rBMSCs and ROBs.HPS-1 significantly increased the area and number of calcified nodules of rBMSCs and ROBs(P<0.01),HPS-2 and HPS-3 had no influence on promoting the formation of calcified nodules of rBMSCs and ROBs.Conclusion HPS-1 could enhance the cell activity and osteogenesis-related factor expression,and significantly promote the differentiation and mineralization of rBMSCs and ROBs cells.